HxSil C18
Silica packing material for a variety of reversed-phase separations Hamilton HxSil C18 columns are well end-capped for retention time reproducibility and durability. HxSil C18 columns exhibit greater retention than most columns and enhances the separation of compounds that are not sufficiently retained on other C18 columns.
Application Examples: Polycyclic aromatic hydrocarbons, small organic molecules.
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HxSil C18 Columns
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HxSil C18 Guard Columns
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HxSil C18 Bulk Resin
Overview of HxSil C18
Alkylated silica is used for reversed-phase HPLC columns. Hamilton offers two varieties of this composition: C18 and C8. C18 columns are more hydrophobic than C8 columns and thereby retain hydrophobic analytes more strongly than a C8 column will. Due to stronger retention, C18 columns tend to result in longer retention times and better separations. C8 columns result in shorter analyte retention times which produce faster analysis times.
How to Choose Column Specifications for Your Application
Tech Center
Specific Applications
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Separation of Eight Cannabinoids by Reversed-Phase HPLC (HxSil C18)
With health, safety, and edibles dosing as the primary motivation, Hamilton Company developed an HPLC method that isolates eight major cannabinoids. The HxSil C18 (3 µm) column provides an accurate, cost effective, and robust solution that can be used in any HPLC system. -
Aflatoxins in Food Sources (HxSil C18)
In this application note we have developed an HPLC method that isolates five different aflatoxins in less than 6 minutes. -
Promoter or Polluter? Separation of Triazines on HxSil C18 Column (HxSil C18)
Triazine herbicides have been found as contaminants in waterways and drinking water supplies.
Resources
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Reference Guide - HPLC Columns
Learn how to choose a column, how to maximize the life of a column through proper care, cleaning, and restoration.
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How do I restore my HPLC column?
Performance will inevitably degrade as the column is used, however this procedure may restore the performance of a column that has become fouled. Learn more in this step-by-step guide. -
How Do I Connect a Stainless Steel Guard Cartridge to My HPLC Column?
The process of connecting a guard cartridge to an HPLC column is fairly simple, but requires different steps based on the HPLC instrument and the guard cartridge configuration. Click to read our step-by-step guide. -
How Do I Install My HPLC Column?
Connecting an chromatography column is not difficult, but if the proper steps aren't followed, chromatographic results can be compromised. Follow this step-by-step guide to get it done right.
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USP "L" Column Listings
Explore Hamilton’s qualifying USP “L” columns. -
HPLC Guard Columns, HPLC Guard Cartridges
HPLC guard columns and guard cartridges protect analytical, semi-prep and preparative HPLC columns. They help by removing particulate contaminants and highly absorptive compounds from samples, prolonging column life.
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