PRP-3 10 µm, 4.1 x 150 mm, PN: 79466

Stainless steel

Part # 79466

PRP-3 (PN: 79466) is based off the PRP-1 resin with surface modification such that it increases the contribution of the large pore sizes. By utilizing a 300 Å pore size, PRP-3 is optimized for the separation of macromolecules.

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PRP-3 10 µm, 4.1 x 150 mm, PN: 79466 Details

Hamilton PRP-3 columns are based on the PRP-1 with a larger pore size for the separation of macromolecules such as DNA, RNA oligos, proteins, peptides, and proteomics. This column resin has no silanol groups, so irreversible protein adsorption is not a concern, giving analytes such as proteins a recovery of > 90%. This stable material also allows the use of concentrated acids, chaotropes, and even detergents to minimize problems with protein solubility.

Application Examples: Organic compounds: large molecules (> 2,000 mw), peptides, proteins, protein digests, protected and deprotected oligonucleotides, nucleic acids

Scientific Citations

PRP-3 10 µm, 4.1 x 150 mm, PN: 79466 Specifications

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Separation Mode Reversed-Phase
Stationary Phase PRP-3
Format HPLC Column
Hardware Inner Diameter 4.1 mm
Hardware Length 150 mm
Particle Size 10 µm
USP Classification USP L21
Packing Material Type PSDVB
Pore Size 300 Å
Particle Surface Area (m2/g) 48
Pore Volume (cm3/g) 0.20
Hardware Material Stainless Steel
Max Pressure 5,000 psi
Mobile Phase Limits pH 1-13, 0-100% aqueous, organic modifier
Buffer Strength 0.0-0.5N
Temp Limits 5-85°C
Restoration Run a gradient of 100% water to 100% acetonitrile. Repeat three times
Compound Classification Peptides/Proteins
UOM 1 EACH

PRP-3 10 µm, 4.1 x 150 mm, PN: 79466 Resources

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

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Application Note

GPCRs - The Biological Traffic Modulator (PRP-3)

The analysis of peptides in the GI tract has revealed connections between these peptides and other body systems, such as the endocrine, cardiac, and central nervous systems. Understanding the relationship between these peptides is essential to understanding the body's balance. Hamilton Company's HPLC stationary phases are critical to isolating GPCR binding peptides.
Separation of Biologically Active Increasing Length Peptides

Application Note

Separation of Biologically Active Increasing Length Peptides (PRP-3)

Biologically active peptides harbor an important role in human health. They are associated with the development of new therapeutic health care products and as potential biologic pathway markers and metabolites in the detection of disease.
HAM SDS PRP 3 resin dry

SDS

SDS - PRP-3 (resin, dry)

Download the Safety Data Sheet for Hamilton PRP-3 Resin (Dry), used as a filter media in HPLC applications. Includes key safety, handling, and first aid information.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

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