HPLC Columns
High-Value HPLC Columns for Every Laboratory
The Right Choice for HPLC Columns with Optimal Peak Performance
High-performance liquid chromatography (HPLC) is one of the most important analytical techniques across the life sciences. Here, components in a chemical mixture are separated, purified, identified, and quantified. Pharmaceutical, toxicology, and so many other industry segments critically depend on HPLC data to support their insights and next steps.
When your lab faces pressure to deliver high-quality data, put your trust in a wide variety of HPLC column types from the decades-long leader in chromatography columns.
Hamilton HPLC Column Benefits
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HPLC Columns
Reversed Phase
Hamilton reversed-phase HPLC columns combine the best characteristics of silica-based and polymeric columns to arrive at a product that is highly inert and long-lasting.
HPLC Columns
Anion Exchange
In anion exchange chromatography, the stationary bed has an ionically positive (+) charged surface while the sample ions are of negative (-) charge. The stronger the negative charge on the sample, the stronger it will be attracted to the positive charge on the stationary phase.
HPLC Columns
Cation Exchange
In cation exchange chromatography, the stationary bed has an ionically negative (-) charged surface while the sample ions are of positive (+) charge. This technique is used almost exclusively with ionic or ionizable samples.
HPLC Columns
Ion Exclusion
Ion exclusion chromatography is an alternative to ion exchange chromatography in which ionized samples are excluded from the pores of the support and elute first, while the weakly ionized and non-ionic compounds elute later.
HPLC Columns
Build Your Own HPLC Column
Can't find the exact Hamilton HPLC Column you're looking for? We can make them to order!
HPLC Columns
Custom HPLC Column Packing Services
Trust Hamilton's experienced chromatography team to expertly pack your analytical to preparative scale HPLC columns.
What are HPLC Columns?
High-performance liquid chromatography (HPLC), is an analytical technique in chemistry used to separate, purify, identify, and quantify each component in a chemical mixture. In HPLC systems, high-pressure pumps force solvents containing a chemical sample mixture through a metal or polymer plastic tube that is filled with a stationary phase in the form of small, spherical particles (typically less than 100 µm in diameter). Each chemical analyte of the sample mixture interacts differently with the stationary particles and flows through the column at different rates of speed, passing through a detector at the end of the column at different times where they are recorded.
Chromatography columns are most commonly employed in the scientific fields of pharmaceutical, biopharmaceutical, environmental, toxicology, food and beverage, etc. Common uses of HPLC include detecting environmental contaminants in soil and groundwater, production of medications, purification of DNA, etc.
Latest Chromatography Application Notes
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HPLC Columns Resources
Browse app notes, user guides, specification documents, and more in our Knowledge Center.
Browse app notes, user guides, specification documents, and more in our Knowledge Center.
Browse app notes, user guides, specification documents, and more in our Knowledge Center.
Browse app notes, user guides, specification documents, and more in our Knowledge Center.
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