HxSil C18

Hamilton HxSil C18

Silica packing material for a variety of reversed-phase separations, Hamilton HxSil C18 columns are well-end-capped for retention time reproducibility and durability. HxSil C18 columns exhibit greater retention than most columns and enhance the separation of compounds that are not sufficiently retained on other C18 columns.

Application Examples: Polycyclic aromatic hydrocarbons, small organic molecules.

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HxSil C18 Columns

End-capped for retention time reproducibility and durability.
HxSil C18 Columns

HxSil C18 Guard Columns

Protect your HxSil C18 column.
HxSil C18 Guard Columns

HxSil C18 Bulk Resin

Pack your own column.
HxSil C18 Bulk Resin

Overview of HxSil C18

Alkylated silica is used for reversed-phase HPLC columns. Hamilton offers two varieties of this composition: C18 and C8. C18 columns are more hydrophobic than C8 columns and thereby retain hydrophobic analytes more strongly than a C8 column will. Due to stronger retention, C18 columns tend to result in longer retention times and better separations. C8 columns result in shorter analyte retention times which produce faster analysis times.

Tech Center

Choosing Column Specifications
HPLC Column Care & Troubleshooting
HPLC Resin Specifications
HPLC Guard Cartridge Holder Assembly - Stainless Steel
Safety Data Sheets

HxSil C18 Resources

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

HAM Reference Guide HPLC Columns

Guide

Reference Guide - HPLC Columns

Learn how to choose a column, how to maximize the life of a column through proper care, cleaning, and restoration.
Food Sources

Application Note

Aflatoxins in Food Sources (HxSil C18)

In this application note we have developed an HPLC method that isolates five different aflatoxins in less than 6 minutes.
HAM SDS Hx Sil C18 resin dry

SDS

SDS - HxSil C18 (resin, dry)

Download the Safety Data Sheet for Hamilton HxSil C18 Resin (Dry), used as a filter media in HPLC applications. Includes key safety, handling, and first aid information.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

cation exchange column

Article

USP "L" Column Listings

Explore Hamilton’s qualifying USP “L” columns.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

HPLC FAQ

FAQ

How Do I Connect a Stainless Steel Guard Column to My HPLC Column?

Connecting a guard column to an HPLC column is straightforward, but requires different steps based on the HPLC instrument and its configuration. Click the headline above to read this step-by-step guide.
Abstract photos of Liquid for Hamilton C18 vs. C8 Article

FAQ

C8 vs C18 Column: Which Should You Choose?

This guide explains how to choose between C8 and C18 HPLC columns for reverse-phase chromatography, comparing their retention, hydrophobicity, and best use cases for method development.
HPLC FAQ

FAQ

How do I restore my HPLC column?

Performance will inevitably degrade as the column is used, however this procedure may restore the performance of a column that has become fouled. Learn more in this step-by-step guide.
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