NEBNext Ultra II Directional RNA Library Preparation Workflows on the NGS STAR

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Application note 2409 12 NEB RNA Workflows TIMG

New England Biolabs Inc. (NEB), the European Molecular Biology Laboratory (EMBL GeneCore), and Hamilton have developed automated versions of the poly(A) mRNA enrichment and rRNA depletion workflows with a seamless transition into the NEBNext Ultra II Directional RNA Library Prep Kit. 

The automation solutions of these protocols cater to the needs of users seeking scalable throughput with minimal hands-on time. The streamlined procedures allow for increased sensitivity and specificity in RNA-Seq experiments by choosing between the removal of interfering rRNAs or the enrichment of mRNAs by oligo d(T) beads.

  • Generate high-quality libraries with a broad range of input amounts
  • Save time with streamlined workflows and maximized walk-away automation
  • Increase sample throughput with high reproducibility

 

 

 

 

 

 

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