Perhaps the most common application in biopharma is the measurement of dissolved oxygen in cell culture applications. Oxygen is a critical process parameter (CPP) required for cell growth. Cells are grown in a vessel called a bioreactor, where oxygen is maintained at approximately 30 to 60% air saturation. Too little oxygen stresses the cells, limits growth (hypoxia) or possibly causes cell death (anoxia). Excessive levels of oxygen cause the negative effects of foam buildup within the vessel and even oxygen toxicity.
DO sensors used in bioreactor applications can be either polarographic or optical. These sensors must have a hygienic design and the ability to withstand sterilization (autoclave or steam-in-place) as well as chemical cleaning (clean-in-place).
Hamilton DO Sensors
To explore specific applications in biopharma please review our Biopharma industry page.
Basic bioreactor layout