Single-Use Skid for LPLC Chromatography
The Role of Low Pressure Liquid Chromatography
Chromatography LPLC is a low-pressure liquid chromatography process that operates under pressures less than 6 bar. This technique allows for the transfer of molecules between a solid stationary phase (known as chromatography media or gel) and a liquid mobile phase, which conditions the stationary phase to desorb the molecules being purified. The stationary phase is located within a chromatography column, which is evenly distributed with the mobile phase from top to bottom by two distributors.
The VERDOT Ips² FlexiPro is a benchtop system adaptable to a wide range of chromatography processes. Inline dilution or gradient of aqueous buffers can be performed with the FlexiPro system. Only the most precise and reliable disposable sensors such as the Hamilton OneFerm or the Conducell 4USF are used in order to monitor the pH and Conductivity of the process and control the flow rate and dilution. Other critical process parame-ters such as Protein titer (UV), flow, and temperature are monitored as well.
LPLC is typically used in the following cases
- Fine separation of “high” value molecules (>2 $/g in order of magnitude).
- Separating molecules of similar size, where filtration can’t work.
- Fragile molecules or cells.
- Purification and concentration, in Bind & Elute mode: valuable molecules can be very diluted in the load solution and end up contained in the bed volume after adsorption. They can be eluted in 2-5 column volumes.
- The change of solvent in Bind & Elute mode can help further purify. Example in Reverse Phase Chromatography: load raw product in water, elute the target molecules in EtOH that can be easily evaporated.
- Adaptable to any kind of molecules: proteins, oligonucleotides, sugars, lipids, and cells (virus).
VERDOT Ips² FlexiPro with Low Flow Kit including flow-cells and pH/Conductivity Sensors
The VERDOT Ips² FlexiPro is a benchtop system adaptable to a wide range of chromatography processes. Inline dilution or gradient of aqueous buffers can be performed with the FlexiPro system. Only the most precise and reliable disposable sensors such as the Hamilton OneFerm or the Conducell 4USF are used in order to monitor the pH and Conductivity of the process and control the flow rate and dilution. Other critical process parame-ters such as Protein titer (UV), flow, and temperature are monitored as well.
Hamilton Products Used
OneFerm - Arc
Conducell 4UsF - Arc
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