How do I install a different adapter onto my HHC?
You may want to use your Hamilton Heater Cooler for a PCR plate or a tube. This article describes how to install the appropriate adapter plate you’ll need for your labware.
Requirements
- HHC plate adapter(s)
- 67391-01: 96-Well PCR Adapter, or
- 67392-01: 384-Well PCR Adapter, or
- 6609979-01: 24-Tube Adapter
- 2 mm Hex wrench (included with ML Prep)
- No. 1 Phillips screwdriver (included with HHC)
- Small flat head screwdriver
Procedure
Ensure the ML Prep is turned off.
- Use a No. 1 Phillips screwdriver to remove the 8 illustrated screws in Figure 1.
Figure 1. Flat Plate adapter and corner locator removal - Remove the corner locators (optional for 24-Tube Adapter).
- Remove the flat plate adapter and store all removed items in a safe place.
Installing a PCR Adapter
There are 2 HHC PCR adapters—a 384-well and a 96-well.
- Peel off the adhesive backing on the 2 provided thermal pads (per adapter). Press the adhesive sides into the holes in the bottom of the adapter to be installed, as seen in Figure 2.
Figure 2. Bottom of PCR adapter - Using a 2 mm Hex wrench, attach the appropriate PCR adapter plate with the 4 screws supplied in the kit.
- Tighten the screws until they are snug, but not overly tight.
Figure 3. 96-well PCR adapter installation
Installing a Tube Adapter
- Using a flat head screwdriver, install the 4 locator pins included with the kit into the holes on the HHC, indicated in Figure 5.
- Tighten the screws until they are snug, but not overly tight.
- Set Tube adapter on HHC.
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