A prerequisite for an accurate pH calibration is the availability of suitable buffer solutions. Hamilton 12.00 DuraCal liquid buffer solution (Ref 238279) has an accuracy of +/- 0.05 pH units and a stability of 24 months. The pH value of the buffer is affected by changing temperature. The effects of temperature from 5 to 50°C is shown below in 1 degree increments.

pH vs. Temperature: Reference table for 12.00 DuraCal buffer

Temp [°C]pH ValueTemp [°C]pH Value
5 12.46 30 11.90
6 12.44 31 11.88
7 12.41 32 11.86
8 12.39 33 11.84
9 12.36 34 11.82
10 12.34 35 11.80
11 12.32 36 11.78
12 12.29 37 11.76
13 12.27 38 11.74
14 12.25 39 11.72
15 12.23 40 11.70
16 12.20 41 11.68
17 12.18 42 11.66
18 12.17 43 11.64
19 12.13 44 11.62
20 12.11 45 11.60
21 12.09 46 11.58
22 12.05 47 11.57
23 12.04 48 11.55
24 12.02 49 11.53
25 12.00 50 11.51
26 11.98
27 11.96
28 11.94
29 11.92