PRP-X500: Methacrylate (SAX) Anion Exchange HPLC Column
PRP-X500 is a superficially porous polymeric anion exchange column designed for the separation, purification and isolation of proteins, peptides and DNA/RNA. The methacrylate polymeric coating of the PRP-X500 provides a more hydrophilic surface, preventing hydrophobic interaction sample losses.
Application Examples: Proteins, peptides, and DNA/RNA.
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PRP-X500 Columns
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PRP-X500 Guard Columns
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PRP-X500 Bulk Resin
PRP-X500 Overview
Fast separations and good sample recovery
PRP-X500 is a superficially porous polymeric anion exchange column designed for the separation, purification and isolation of proteins, peptides and DNA/RNA. The methacrylate polymeric coating of the PRP-X500 provides a more hydrophilic surface, preventing hydrophobic interaction sample losses typically seen on other commercially available protein HPLC columns.
The non-porous nature of the packing material improves mass-transfer, shortening run times and improving resolution. Both fast separations and good sample capacity are achievable with PRP-X500 columns.
Sample recovery is excellent with PRP-X500 and the support’s limited permeability prevents proteins from entering the pores and unfolding, which causes peak ghosting. The superficially porous properties of the packing material improves mass-transfer, shortening the diffusion path of the analyte. This results in shortened run times, improved resolution, and sharp sample bands.
How to Choose Column Specifications for Your Application
Tech Center
Weiterführende Informationen
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Reference Guide - HPLC Columns
Learn how to choose a column, how to maximize the life of a column through proper care, cleaning, and restoration.
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How do I restore my HPLC column?
Performance will inevitably degrade as the column is used, however this procedure may restore the performance of a column that has become fouled. Learn more in this step-by-step guide. -
How Do I Connect a Stainless Steel Guard Cartridge to My HPLC Column?
The process of connecting a guard cartridge to an HPLC column is fairly simple, but requires different steps based on the HPLC instrument and the guard cartridge configuration. Click to read our step-by-step guide. -
How Do I Install My HPLC Column?
Connecting an chromatography column is not difficult, but if the proper steps aren't followed, chromatographic results can be compromised. Follow this step-by-step guide to get it done right.
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