Enhancing NGS Library Preparation Efficiency with the QIAseq FX DNA Library Protocol.

Library preparation is a key requirement for Next-Generation Sequencing (NGS) applications and is among the most expensive segments of the sequencing workflow. It is not only a time-consuming step but can also result in sample loss or lower quality of the output library DNA due to handling errors. To reduce these issues, the streamlined QIAseq FX DNA Library protocol is optimized to perform adapter ligation directly after enzymatic fragmentation without the need for an intermediary clean-up step. Furthermore, the straightforward protocol (consisting of only three steps) ensures the smooth library preparation automation of high-priority samples and low-throughput sample numbers on the Hamilton NGS STARlet.