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Immunoassays are one of the most common applications in Clinical Chemistry, and Immunosuppressants are one of these applications. Today’s laboratories are challenged with delivering high standards of laboratory services with fewer resources, so automating the full workflow is key, in order to deliver highly reliable results in the requested time. In particular, the sample pre-treatment is generally a bottleneck that requires several tedious pipetting steps that can leave the laboratory exposed to mistakes. In this Application Note automation of the sample pre-treatment for Immunosuppressant analysis on Roche Cobas e 411 is described in detail.