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Yeast and Bacterial Replication with Pin-Tools


Yeast and bacterial culture replication is an important step in many molecular biology applications. For this purpose, yeast or bacterial colonies are either grown in liquid growth media or on agar plates. Following growth, replication is often done in a highly parallel way using 96 or 384 floating pin tools from V&P Scientific. These tools can easily be handled on the Microlab STARlet. The floating pin tools (with 96 or 384 pins) are precisely moved with the internal CO-RE gripping tool.

Before yeast or bacterial colonies are replicated from one plate to another, the pins are pre-wetted with sterile water. After replication, the floating pin tools are transported to 4 different cleaning positions: cleaning solution, sterile water, 75% ethanol and 100% ethanol. Between the 4 cleaning positions, the pin tool is transported to the different blotting stations to avoid mixing the different solutions. Finally, the pin tool is moved to the heating station where the pins are completely dried within 30sec. The floating pin tool is now ready for the next replication of yeast or bacteria colonies without any contamination.

Features of Automation


Benefits of Automation

  • Microlab STARlet, 4 channels
  • CO-RE Grip for easy plate handling and transport of the Floating Pin Tool
  • Dry Station for Floating Pin Tool (for 96 or 384 pins)
  • Carriers with 4 cleaning positions, 4 blotting modules
  • One carrier with positions for source and destination plates and one pin wetting station
  • Floating Pin Tool with 96 FP6 pins (diameter 1.58mm) from VP Scientific
  • Floating Pin Tool with 384 FP4 pins (diameter 0.914mm) from VP Scientific
  • Microlab SWAP 420, external plate handler for integration of shelves
  • 8 shelves, each with positions for 8 MTP’s
  • Replication of colonies in 96 or 384 well format is possible with different floating pins on the same system
  • Cleaning and sterilization procedure for pin tools prevents contamination
  • Replication of liquid cultures and agar cultures is possible
  • Very low running costs, as no disposable tips are used for the replication