PRP-X300 Columns

Hamilton PRP-X300 columns offer an easy, rapid way to separate closely related alcohols and organic acids. The sulfonated poly(styrene-divinylbenzene) support separates samples via a mixed mode mechanism. Separation on the PRP-X300 is accomplished by three modes:

• Hydrogen Bonding—The attraction and retention of sample compounds by the negatively charged sulfonate group.

• Reversed-Phase—The interaction and retention of the sample compounds by the non-polar polymeric support.

• Ion Exclusion—The process in which ionized samples are excluded from the pores of the support and elute first, while the weakly ionized and non-ionic compounds elute later.