Understanding the binding structure of compounds towards the protein backbone is critical in lead optimization. These efforts seek to identify and characterize proteins in high numbers as well as investigate the structure of proteins and their interactions with one another or with organic compounds. Protein crystallography is a powerful tool that scientists use to unravel this puzzle.
The crystallization of proteins is a multi-factorial process that depends on the interplay of several independent parameters, such as temperature, protein concentration, crystallization agent concentration, and the presence and nature of impurities or additives. To determine the optimal crystallization conditions, special screens for testing various conditions can be developed, and ready-mixed precipitate solutions can be applied.