Autoloader I-III

What is the accuracy of Hamilton syringes?
Can Hamilton syringes be used on humans?
Is the volume of fluid in the needle part of the total volume of the dispensed fluid?
Are the plungers for the 700 series Microliter syringes interchangeable?
What are the most versatile syringes made by Hamilton?
How can I sterilize my syringe?
How do I select the correct needle?
Is the digital syringe automated or manual?
What does the "s" mean in a 22s or 26s gauge needle?
Which rheostat is recommended to work with the syringe cleaner?

What is the accuracy of Hamilton syringes?
Hamilton syringes are manufactured to be accurate within ±1% of nominal volume, and with precision within 1%, measured at 80% of total scale volume. The design of our barrel and plunger dimensions assures high levels of accuracy and precision. The Hamilton Company Quality System is ISO 9001-2008 certified.

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Can Hamilton syringes be used on humans?
Hamilton syringes are not manufactured for human use. To discuss potential off label uses for Hamilton syringes please Contact Us.

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Is the volume of fluid in the needle part of the total volume of the dispensed fluid?
When using a Hamilton syringe, the volume in the needle (called dead volume) is considered constant and the volume being dispensed is absolute. The same dead volume is maintained throughout aspirate and dispense stages.

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Are the plungers for the 700 series Microliter syringes interchangeable?
No, they are not! Each plunger is fitted to a specific syringe barrel. Be very careful to keep each plunger with its original syringe in order to maximize syringe performance.

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What are the most versatile syringes made by Hamilton?
Gastight syringes are the most versatile syringes. Plungers are replaceable if they get bent. Any of the Metal hub, Kel-F or Removable needle allow for the replacement of needle if they become bent or plugged.

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How can I sterilize my syringe?

Chemical sterilization is the preferred method for all Hamilton products. Autoclaving syringes should only be used as a last resort and only where specifically stated.
Syringe sterilization is not straightforward. Not all syringes can be autoclaved. Click here for a list of Hamilton products and their preferred sterilization method.

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How do I select the correct needle?

You will need to answer the following questions:

1. What is your application?
Chromatography, Life Science, etc and what instrument are you using?

2. Do you already have a syringe, if so what kind?
What volume and what termination? Cemented, RN, TLL etc

3. What gauge do you think you need?
We make 10 gauge all the way down to 34 gauge.

4. What length do you need?
Our standard length is 2 inches and all other lengths are considered a custom product
Hamilton makes thousands of different needles, which is why we need as much information from the enduser as possible.

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Is the Digital Syringe automated or manual?
The Digital Syringe is a manual syringe with a digital display which allows you to clearly see the aspirated volume. The Digital Syringe is not automated, it will not dispense with the touch of a button. It operates like a typical syringe, but there is no guessing as to the volume being aspirated or dispensed.

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What does the "s" mean in a 22s or 26s gauge needle?

The "s" represents a smaller inner diameter for the needle and a thicker needle wall for better durability. For example, a 26 gauge needle has an outer diameter of 0.46 mm and an inner diameter of 0.26 mm while the 26s gauge needle has an outer diameter of 0.47 mm and an inner diameter of 0.13 mm. The 26s has half the inner diameter of the 26 gauge needle. The difference in the wall thickness also nearly doubled with 26s gauge having a thickness of 0.18 mm while the 26 gauge is only 0.10 mm.

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Which rheostat is recommended to work with the syringe cleaner?

The syringe cleaner requires a Variable Autotransformer and we recommended finding the rheostat at an electronics supply house like Newark Electronics. For example: Newark Electronics - P/N 66F3591 or Tenma P/N 72-110

Syringe Selection Guide

Download Guide to Maintaining and Using Hamilton Syringes

How can I determine the accuracy of my Hamilton syringe?
I keep bending the plungers on my syringes. How can I prevent this?
The plunger in my syringe is stuck. What can I do to free it up?
Which Hamilton syringe do I have?
Why won't my new removable needles fit into my removable needle syringe?

How can I determine the accuracy of my Hamilton syringe?
Hamilton has developed a gravimetric method for determining the accuracy of our syringes. Click here for the procedure.

I keep bending the plungers on my syringes. How can I prevent this?
The plungers, especially for the smaller volumes bend very easily if not aligned properly or if they are depressed at an angle. To combat this very common problem Hamilton has developed several different products:

For the 700 series, reinforced plungers are available in the 600, 800, 900 series syringes.
For the 1700 series, reinforced plungers are available in the 1800 series. (Reinforced plungers are more cost effective for users who often bend plungers.)

Also available are syringe guides designed to maintain proper plunger alignment. As well as reproducibility guides, which serve two purposes:

Help increase the precision of the injections
Guide the plunger and maintain plunger alignment

The last option to save a syringe with a bent plunger is to buy a Gastight syringe. The plungers can be replaced at a fraction of the cost of a new syringe.

The plunger in my syringe is stuck. What can I do to free it up?
Frozen plungers are caused by improper care of your syringe. Here are a couple of suggestions to save your syringe:

Click here for syringe cleaning and maintenance tips.

Soak the syringe in alcohol, acetone or warm water. Do not soak them for longer than 5 minutes.
Sonic cleaners can sometimes help to free up the plunger.

If the plunger does not start to move after this, you will need to replace the syringe.

Proper cleaning will help prevent frozen plungers. Especially when the syringes are being used for life science applications, where sugary or protein based sample are required. We recommend using a like solvent to dissolves the sample. Rinse the syringe thoroughly and then remove the plunger and allow the syringe and plunger to air dry separately.

Which Hamilton syringe do I have?
Hamilton Company manufactures thousands of different syringes and many of those syringes have the same model number. (the model number is listed on the syringe barrel and is a 3 or 4 digit number, 1701, 1001 or 7001) Because we make multiple variations of each model, it is very important to save the original packaging or at least the original part number for when you need to reorder.

Why won't my new removable needles fit into my removable needle syringe?
All removable needles have a white PTFE ferrule and the ferrule must be removed before the replacement needle can be installed. Generally, the ferrule will come out along with the needle but sometimes it remains behind. If the ferrule does not come out, here are a couple of procedures you can try to get the ferrule out:

Push the plunger all the way through, depending on the size the syringe the ferrule should pop right out.
Hold the needle at an angle as you pull out the needle, the end of the needle will help to pull the ferrule out.

	Download Guide to Selecting the Right Hamilton Syringe for Your Application
	Download Guide to Maintaining and Using Hamilton Syringes

When selecting a needle gauge it is important to keep in mind the volume of the syringe and the dead volume of the needle. For example, it will be very difficult to prime a 10 µL syringe if the dead volume in the needle is greater than 10 µL. Refer to the gauge index to choose a needle gauge with an appropriate µL/inch before selecting a needle. Select the minimum length that allows you to carry out your application comfortably.

Note: The ‘s’ on a 22s needle represents a smaller I.D. (inner diameter) for the needle and a thicker needle wall for better durability. For example, a 26 gauge needle has an O.D. (outer diameter) of 0.46 mm and an I.D. of 0.26 mm while the 26s gauge needle has an O.D. of 0.47 mm and an I.D. of 0.13 mm. The 26s has half the I.D. of the 26 gauge needle. Also, the difference in the wall thickness nearly doubles with 26s gauge having a thickness of 0.18 mm while the 26 gauge is only 0.10 mm.

	Nominal O.D.		Nominal I.D.		Wall Thickness		Volume
Gauge	Inch	mm*	Inch	mm*	Inch	mm*	µL/Inch
34	0.0060 - 0.0065	0.159	0.0015 - 0.0025	0.051	0.0020	0.051	0.052
33	0.0080 - 0.0085	0.210	0.0035 - 0.0050	0.108	0.0020	0.051	0.233
32	0.0090 - 0.0095	0.235	0.0035 - 0.0050	0.108	0.0025	0.064	0.233
31	0.0100 - 0.0105	0.261	0.0045 - 0.0060	0.133	0.0025	0.064	0.353
30	0.0120 - 0.0125	0.312	0.0055 - 0.0070	0.159	0.0030	0.076	0.504
29	0.0130 - 0.0135	0.337	0.0065 - 0.0080	0.184	0.0030	0.076	0.675
28	0.0140 - 0.0145	0.362	0.0065 - 0.0080	0.184	0.0035	0.089	0.675
27	0.0160 - 0.0165	0.413	0.0075 - 0.0090	0.210	0.0040	0.102	0.876
26s	0.0184 - 0.0189	0.474	0.0045 - 0.0055	0.127	0.0070	0.178	0.322
26	0.0180 - 0.0185	0.464	0.0095 - 0.0110	0.260	0.0040	0.102	1.349
25s	0.0200 - 0.0205	0.515	0.0055 - 0.0065	0.153	0.0070	0.178	0.464
25	0.0200 - 0.0205	0.515	0.0095 - 0.0110	0.260	0.0050	0.127	1.349
24	0.0220 - 0.0225	0.566	0.0115 - 0.0130	0.311	0.0050	0.127	1.930
23s	0.0250 - 0.0255	0.642	0.0040 - 0.0051	0.116	0.0105	0.267	0.268
23	0.0250 - 0.0255	0.642	0.0125 - 0.0140	0.337	0.0060	0.152	2.266
22s	0.0280 - 0.0285	0.718	0.0055 - 0.0077	0.168	0.0110	0.279	0.563
22	0.0280 - 0.0285	0.718	0.0155 - 0.0170	0.413	0.0060	0.152	3.403
21	0.0320 - 0.0325	0.819	0.0195 - 0.0210	0.514	0.0060	0.152	5.271
20	0.0355 - 0.0360	0.908	0.0230 - 0.0245	0.603	0.0060	0.152	7.255
19	0.0415 - 0.0425	1.067	0.0255 - 0.0285	0.686	0.0075	0.191	9.389
18	0.0495 - 0.0505	1.270	0.0315 - 0.0345	0.838	0.0085	0.216	14.011
17	0.0575 - 0.0585	1.473	0.0405 - 0.0435	1.067	0.0080	0.203	22.715
16	0.0645 - 0.0655	1.651	0.0455 - 0.0485	1.194	0.0090	0.229	28.444
15	0.0715 - 0.0725	1.829	0.0525 - 0.0555	1.372	0.0090	0.229	37.529
14	0.0820 - 0.0840	2.109	0.0610 - 0.0650	1.600	0.0100	0.254	51.076
13	0.0940 - 0.0960	2.413	0.0690 - 0.0730	1.804	0.0120	0.305	64.895
12	0.1080 - 0.110	2.769	0.0830 - 0.0870	2.159	0.0120	0.305	93.000
11	0.1190 - 0.1210	3.048	0.0920 - 0.0960	2.388	0.0130	0.330	113.728
10	0.1330 - 0.1350	3.404	0.1040 - 0.1080	2.693	0.0140	0.356	144.641

*mm are nominal

7000 Series Modified Microliter Syringes

Volume		0.5 µL	0.5 µL	1 µL	1 µL	2 µL	2 µL	5 µL
Model		7000.50C	7000.5	7001	7101	7002	7102	7105
Scale length, mm		60	60	60	60	60	60	60
Total scale divisions		100	100	100	100	100	100	100
Major graduations, µL		0.05	0.05	0.1	0.1	0.2	0.2	0.5
Minor graduations, µL		0.01	0.01	0.02	0.02	0.04	0.04	0.1
Sub-minor graduations, µL		0.005	0.005	0.01	0.01	0.02	0.02	0.05
Maximum temperature	°C	115	115	115	115	115	115	115
Maximum temperature	°F	239	239	239	239	239	239	239
Minimum temperature	°C	10	10	10	10	10	10	10
Minimum temperature	°F	50	50	50	50	50	50	50
Maximum pressure rating	bars	414	414	414	414	414	414	414
Maximum pressure rating	psig	6000	6000	6000	6000	6000	6000	6000
Standard needle gauge		32	25	25	22	25	23	24
Standard needle outer diameter (O.D.)	mm	0.235	0.515	0.515	0.718	0.515	0.642	0.566
Standard needle outer diameter (O.D.)	inches	0.0093	0.0203	0.0203	0.0283	0.0203	0.0253	0.0223
Standard needle inner diameter (I.D.)	mm	0.104	0.104	0.145	0.145	0.206	0.257	0.325
Standard needle inner diameter (I.D.)	inches	0.0041	0.0041	0.0057	0.0057	0.0081	0.0081	0.0128
Standard needle length	mm	100	70	70	70	70	70	70
Standard needle length	inches	3.94	2.75	2.75	2.75	2.75	2.75	2.75
Standard electro-taper		No	No	No	Yes	No	Yes	No
Standard needle dead volume, µL		0	0	0	0	0	0	0

600 Series Microliter Syringes

Volume		2.5 µL	5 µL
Model		62	65
Scale length, mm		27	27
Total scale divisions		50	50
Major graduations, µL		0.5	1.0
Minor graduations, µL		0.1	0.2
Sub-minor graduations, µL		0.05	0.1
Maximum temperature	°C	115	115
Maximum temperature	°F	239	239
Minimum temperature	°C	10	10
Minimum temperature	°F	50	50
Maximum pressure rating	bars	138	138
Maximum pressure rating	psig	2000	2000
Standard needle gauge		22s	22s
Standard needle outer diameter (O.D.)	mm	0.718	0.718
Standard needle outer diameter (O.D.)	inches	0.0283	0.0283
Standard needle inner diameter (I.D.)	mm	0.168	0.168
Standard needle inner diameter (I.D.)	inches	0.0066	0.0066
Standard needle length	mm	51	51
Standard needle length	inches	2	2
Standard electro-taper		No	No
Standard needle dead volume, µL		1.13	1.13

700 Series Microliter Syringes

Volume		5 µL	10 µL	25 µL	50 µL	100 µL	250 µL	500 µL

Model		75	701	702	705	710	725	750
Scale length, mm		54	54	60	60	60	60	60
Total scale divisions		100	100	100	100	100	100	100
Major graduations, µL		0.5	1.0	2.5	5.0	10	25	50
Minor graduations, µL		0.1	0.2	0.5	1.0	2.0	5.0	10
Sub-minor graduations, µL		0.05	0.1	0.25	0.5	1.0	2.5	5.0
Maximum temperature*	°C	50	50	50	50	50	50	50
Maximum temperature*	°F	122	122	122	122	122	122	122
Maximum temperature	°C	115	115	115	115	115	115	115
Maximum temperature	°F	239	239	239	239	239	239	239
Minimum temperature	°C	10	10	10	10	10	10	10
Minimum temperature	°F	50	50	50	50	50	50	50
Maximum pressure rating	bars	138	138	138	138	138	69	69
Maximum pressure rating	psig	2000	2000	2000	2000	2000	1000	1000
Standard needle gauge		26s	26s	22s	22s	22s	22s	22
Standard needle outer diameter (O.D.)	mm	0.474	0.474	0.718	0.718	0.718	0.718	0.718
Standard needle outer diameter (O.D.)	inches	0.0187	0.0187	0.0283	0.0283	0.0283	0.0283	0.0283
Standard needle inner diameter (I.D.)	mm	0.127	0.127	0.168	0.168	0.168	0.168	0.413
Standard needle inner diameter (I.D.)	inches	0.0050	0.0050	0.0066	0.0066	0.0066	0.0066	0.0163
Standard needle length	mm	51	51	51	51	51	51	51
Standard needle length	inches	2	2	2	2	2	2	2
Standard electro-taper		Yes	Yes	Yes	Yes	Yes	Yes	Yes
Standard needle dead volume, µL		0.64	0.64	1.13	1.13	1.13	1.13	6.81

*N or LTN Cemented Needle or Luer Tip Cemented Needle syringes only

800 Series Microliter Syringes

Volume		5 µL	10 µL	25 µL	50 µL	100 µL	250 µL
Model		85	801	802	805	810	825
Scale length, mm		54	54	60	60	60	60
Total scale divisions		100	100	100	100	100	100
Major graduations, µL		0.5	1.0	2.5	5.0	10	25
Minor graduations, µL		0.1	0.2	0.5	1.0	2.0	5.0
Sub-minor graduations, µL		0.05	0.1	0.25	0.5	1.0	2.5
Maximum temperature*	°C	50	50	50	50	50	50
Maximum temperature*	°F	122	122	122	122	122	122
Maximum temperature	°C	115	115	115	115	115	115
Maximum temperature	°F	239	239	239	239	239	239
Minimum temperature	°C	10	10	10	10	10	10
Minimum temperature	°F	50	50	50	50	50	50
Maximum pressure rating	bars	138	138	138	138	138	138
Maximum pressure rating	psig	2000	2000	2000	2000	2000	2000
Standard needle gauge		26s	26s	22s	22s	22s	22s
Standard needle outer diameter (O.D.)	mm	0.474	0.474	0.718	0.718	0.718	0.718
Standard needle outer diameter (O.D.)	inches	0.0187	0.0187	0.0283	0.0283	0.0283	0.0283
Standard needle inner diameter (I.D.)	mm	0.127	0.127	0.168	0.168	0.168	0.168
Standard needle inner diameter (I.D.)	inches	0.0050	0.0050	0.0066	0.0066	0.0066	0.0066
Standard needle length	mm	51	51	51	51	51	51
Standard needle length	inches	2	2	2	2	2	2
Standard electro-taper		Yes	Yes	Yes	Yes	Yes	Yes
Standard needle dead volume, µL		0.64	0.64	1.13	1.13	1.13	1.13

*N or LTN Cemented Needle or Luer Tip Cemented Needle syringes only

900 Series Microliter Syringes

Volume		5 µL	10 µL
Model		95	901
Scale length, mm		54	54
Total scale divisions		100	100
Major graduations, µL		0.5	1.0
Minor graduations, µL		0.1	0.2
Sub-minor graduations, µL		0.05	0.1
Maximum temperature*	°C	50	50
Maximum temperature*	°F	122	122
Maximum temperature	°C	115	115
Maximum temperature	°F	239	239
Minimum temperature	°C	10	10
Minimum temperature	°F	50	50
Maximum pressure rating	bars	138	138
Maximum pressure rating	psig	2000	2000
Standard needle gauge		26s	26s
Standard needle outer diameter (O.D.)	mm	0.474	0.474
Standard needle outer diameter (O.D.)	inches	0.0187	0.0187
Standard needle inner diameter (I.D.)	mm	0.127	0.127
Standard needle inner diameter (I.D.)	inches	0.0050	0.0050
Standard needle length	mm	51	51
Standard needle length	inches	2	2
Standard electro-taper		Yes	Yes
Standard needle dead volume, µL		0.64	0.64

*N or LTN Cemented Needle or Luer Tip Cemented Needle syringes only

1000 Series Gastight Syringes

Volume		1 mL	1.25 mL	2.5 mL	5 mL	10 mL	25 mL	50 mL	100 mL
Model		1001	1001.25	1002	1005	1010	1025	1050	1100
Scale length, mm		60	60	60	60	60	60	60	120
Total scale divisions		100	100	100	100	100	100	100	200
Major graduations, mL		0.1	0.125	0.25	0.5	1.0	2.5	5.0	5.0
Minor graduations, mL		0.02	0.025	0.05	0.1	0.2	0.5	1.0	1.0
Sub-minor graduations, mL		0.01	0.0125	0.025	0.05	0.1	0.25	0.5	0.5
Maximum temperature*	°C	50	50	50	50	50	50	50	50
Maximum temperature*	°F	122	122	122	122	122	122	122	122
Maximum temperature	°C	115	115	115	115	115	80	80	80
Maximum temperature	°F	239	239	239	239	239	176	176	176
Minimum temperature	°C	10	10	10	10	10	10	10	10
Minimum temperature	°F	50	50	50	50	50	50	50	50
Maximum pressure rating	bars	14	14	14	14	14	7	7	7
Maximum pressure rating	psig	200	200	200	200	200	100	100	100
Standard needle gauge		22	22	22	22	22
Standard needle outer diameter (O.D.)	mm	0.718	0.718	0.718	0.718	0.718
Standard needle outer diameter (O.D.)	inches	0.0283	0.0283	0.0283	0.0283	0.0283
Standard needle inner diameter (I.D.)	mm	0.413	0.413	0.413	0.413	0.413
Standard needle inner diameter (I.D.)	inches	0.0163	0.0163	0.0163	0.0163	0.0163
Standard needle length	mm	51	51	51	51	51
Standard needle length	inches	2	2	2	2	2
Standard electro-taper		Yes	Yes	Yes	Yes	Yes
Standard needle dead volume, µL		6.81	6.81	6.81	6.81	6.81

*N or LTN Cemented Needle or Luer Tip Cemented Needle syringes only.

1700 Series Gastight Syringes

Volume		10 µL	25 µL	50 µL	100 µL	250 µL	500 µL
Model		1701	1702	1705	1710	1725	1750
Scale length, mm		60	60	60	60	60	60
Total scale divisions		100	100	100	100	100	100
Major graduations, µL		1.0	2.5	5.0	10	25	50
Minor graduations, µL		0.2	0.5	1.0	2.0	5.0	10
Sub-minor graduations, µL		0.1	0.25	0.5	1.0	2.5	5.0
Maximum temperature*	°C	50	50	50	50	50	50
Maximum temperature*	°F	122	122	122	122	122	122
Maximum temperature	°C	115	115	115	115	115	115
Maximum temperature	°F	239	239	239	239	239	239
Minimum temperature	°C	10	10	10	10	10	10
Minimum temperature	°F	50	50	50	50	50	50
Maximum pressure rating	bars	69	69	69	69	34	48
Maximum pressure rating	psig	1000	1000	1000	1000	500	700
Standard needle gauge		26s	22s	22s	22s	22s	22
Standard needle outer diameter (O.D.)	mm	0.474	0.718	0.718	0.718	0.718	0.718
Standard needle outer diameter (O.D.)	inches	0.0187	0.0283	0.0283	0.0283	0.0283	0.0283
Standard needle inner diameter (I.D.)	mm	0.127	0.168	0.168	0.168	0.168	0.413
Standard needle inner diameter (I.D.)	inches	0.0050	0.0066	0.0066	0.0066	0.0066	0.0163
Standard needle length	mm	51	51	51	51	51	51
Standard needle length	inches	2	2	2	2	2	2
Standard electro-taper		Yes	Yes	Yes	Yes	Yes	Yes
Standard needle point style		2	2	2	2	2	2
Standard needle dead volume, µL		0.64	1.13	1.13	1.13	1.13	6.81

*N or LTN Cemented Needle or Luer Tip Cemented Needle syringes only

1800 Series Gastight Syringes

Volume		10 µL	25 µL	50 µL	100 µL	250 µL
Model		1801	1802	1805	1810	1825
Scale length, mm		60	60	60	60	60
Total scale divisions		100	100	100	100	100
Major graduations, µL		1.0	2.5	5.0	10	25
Minor graduations, µL		0.2	0.5	1.0	2.0	5.0
Sub-minor graduations, µL		0.1	0.25	0.5	1.0	2.5
Maximum temperature*	°C	50	50	50	50	50
Maximum temperature*	°F	122	122	122	122	122
Maximum temperature	°C	115	115	115	115	115
Maximum temperature	°F	239	239	239	239	239
Minimum temperature	°C	10	10	10	10	10
Minimum temperature	°F	50	50	50	50	50
Maximum pressure rating	bars	69	69	69	69	34
Maximum pressure rating	psig	1000	1000	1000	1000	500
Standard needle gauge		26s	22s	22s	22s	22s
Standard needle outer diameter (O.D.)	mm	0.474	0.718	0.718	0.718	0.718
Standard needle outer diameter (O.D.)	inches	0.0187	0.0283	0.0283	0.0283	0.0283
Standard needle inner diameter (I.D.)	mm	0.127	0.168	0.168	0.168	0.168
Standard needle inner diameter (I.D.)	inches	0.0050	0.0066	0.0066	0.0066	0.0066
Standard needle length	mm	51	51	51	51	51
Standard needle length	inches	2	2	2	2	2
Standard electro-taper		Yes	Yes	Yes	Yes	Yes
Standard needle dead volume, µL		0.64	1.13	1.13	1.13	1.13

*N or LTN Cemented Needle or Luer Tip Cemented Needle syringes only

Super Syringes

Volume		500 mL	1000 mL	1500 mL	2000 mL
Model		S50500	S1000	S1500	S2000
Scale length, mm		166	167	250	334
Total scale divisions		25	50	75	100
Major graduations, mL		100	100	100	100
Minor graduations, mL		20	20	20	20
Maximum temperature*	°C	80	80	80	80
Maximum temperature*	°F	176	176	176	176
Minimum temperature	°C	0	0	0	0
Minimum temperature	°F	32	32	32	32
Maximum pressure rating	bars	2	2	2	2
Maximum pressure rating	psig	30	30	30	30

Terminations are located at the end of the syringe barrel and function as the interface between the syringe and its mating connection such as the needle. Terminations are offered in a number of different needle and connection configurations to accommodate a broad range of applications.

Below is a listing of the most popular syringe terminations.

		N, Cemented Needle For low volume syringes the needles are cemented into the glass syringe barrel at a point corresponding to the zero graduation mark. With this termination, dead volume is limited to the internal volume of the needle. Not autoclavable. Needle gauge is determined by the syringe volume and are not user-selectable.
		SN, Special Cemented Needle For low volume syringes the special needle terminations are the same as the Cemented Needle terminations except they allow for a variety of user-defined gauges, lengths and point styles to be attached.
		LTN, Luer Tip Cemented Needle For mid volume syringes the needles are cemented into the glass syringe barrel at a point corresponding to the zero graduation mark. With this termination, dead volume is limited to the internal volume of the needle. Not autoclavable. Needle gauge is determined by the syringe volume and are not user-selectable.
		LTSN, Luer Tip Special Cemented Needle For mid volume syringes the special needle terminations are the same as the Luer Tip Cemented Needle terminations except they allow for a variety of user-defined gauges, lengths and point styles to be attached.
		LT, Luer Tip The needles are removable and fit over a ground glass hub which is tapered in the shape of a male luer. The LT termination will accept most hypodermic needles but was designed specifically for use with Hamilton Kel-F needles. This termination increases the dead volume in the syringe, which may not be appropriate for some applications. Autoclavable when disassembled.
		RN, Removable Needle The needles are removable and are a Hamilton-specific design. The design allows the needles to seat precisely at the zero graduation mark of the syringe. Users can select the needle gauge, length and point style to optimize the syringe for custom applications. Additionally, this termination allows for a removable needle without increasing the dead volume of the syringe and is ideal when there is a risk of the needle clogging. Autoclavable when disassembled. Repeated autoclaving will shorten syringe life.
		KH, Knurled Hub The knurled hub is used exclusively on 7000 Series syringes. The hub handles up to 6000 psig maximum injection pressure. The needle is removable but with a limited number of gauges available because the plunger is fitted inside the needle. Autoclavable when disassembled. Repeated autoclaving will shorten syringe life.
		FN, Fixed Needle This termination is found on CTC chromatography syringes. The unique design features a direct attachment of the needle to the barrel and eliminates contact between the sample solvent and the adhesive reducing carry-over.
		TLL, PTFE^® Luer Lock This termination has a PTFE, male Luer taper with nickel-plated brass locking hub for use with Kel-F needles, metal hub needles and universal connectors. Also, the TLL is used with Hamilton Diluters/Dispensers, OEM applications and manual operations. Autoclavable when disassembled, except on 25 mL and greater syringes. Repeated autoclaving will shorten syringe life.
		BFP, Bubble Free Prime This syringe is used on syringe pumps like the Microlab 600 Diluters/Dispensers conical plunger tip to flush all liquid from this termination. The resulting syringe is quicker to prime and flush during washes or solvent changes.
		SL, SampleLock™ The SampleLock incorporates an On/Off syringe valve with RN needle. This termination is used for headspace, environmental sample collection and storage, pre-pressurization of gaseous samples for GC analysis, and sample spiking. Not autoclavable.
		C, ChemSeal The ChemSeal termination features a ¼-28 UNF male fitting. This syringe is used in low volume applications where system dead volume needs to be minimized. These syringes can be screwed directly into Hamilton HV, HVP, HVX valves.
		CA, Carbon Analyzer This syringe is used for water analysis with total organic carbon (TOC) analyzers. The termination is designed to minimize the chance of organic carbon contamination from the needle connection.
		DX, Diluter with Stop This is a PTFE Luer Lock Male fitting with an M6 female side port. These syringes attach to instrumentation such as the Microlab 500 series diluters and dispensers.
		DAD, Diluter AccuDil These syringes have an axial fine thread M8 x 0.75 and an M6 female side port. These syringes attach to instrumentation such as the Microlab 1000 series diluters and dispensers.
		AD, AccuDil These syringes have an axial fine thread M8 x 0.75. These syringes attach to instrumentation such as the Microlab 1000 series Diluters/Dispensers.
		Tracheal This termination accepts 5/8" I.D. flexible tubing. These syringes are used for air sampling, preparing gas standards, calibrating reservoirs and pneumographs.

Microliter Plunger

This is the standard, stainless steel plunger found in 2.5 – 500 µL volume Hamilton Microliter syringes. This plunger is hand fitted to the glass barrel to a tolerance of millionths of an inch to create a liquid tight seal ideal for dispensing solvents. When using samples without dissolved particles, the Microliter plunger has an almost unlimited lifespan because there are no wear surfaces. Also, these plungers are not interchangeable between syringes or replaceable due to the custom manufacturing process.
Hamilton Microliter Plunger

7000 Series Microliter Plunger

This plunger is exclusively for 0.5 – 5 µL volume 7000 series syringes. The end of the plunger has a tungsten wire extension that fits the entire length of the needle all the way to the tip, resulting in zero dead volume. This 7000 series syringe is used for dispensing minute liquid volumes.

Hamilton 7000 Series Plunger

Gastight Plungers

Hamilton manufactures a variety of Gastight plungers depending on the intended use of the syringe. All Gastight plungers are made of stainless steel or aluminum and feature a polymer plunger tip, often PTFE. These plungers can be used for manual or automated applications with liquids or gases. The plungers are interchangeable and replaceable for 10 µL – 100 mL volume syringes.
Hamilton Gastight Plunger

Manual Plungers

Manual plungers come in two designs, one features a standard dome button and the other features a threaded plunger button. Syringes smaller than 1 mL have a stainless steel plunger for syringes, as shown in the picture on the left. Manual plungers 1 mL and larger are coated with PTFE and come with a threaded hole that allows them to be mounted into automated syringe pumps, which is demonstrated by the image on the right.
Hamilton Manual Plunger

X-Style Plunger

This plunger is used for syringes 500 µL and smaller intended for use in an automated syringe pump. A special plunger button protects the delicate plunger wire by stopping the pump at the zero line before damaging the tip. The stop also provides a 6-32 threaded hole that is used to mount the plunger into the syringe drive.
Hamilton X Style Plunger

XP-Style Plunger

This plunger is used on XP-3000 modular digital syringe pumps and has a stop to limit damage to the plunger tip. This plunger is for half-height syringes with a 30 mm stroke length.
Hamilton C XP Plunger

XL-Style Plunger

This plunger is used on the XL-3000 modular digital syringe pumps and has a stop to limit damage to the plunger tip. This plunger is for full-height syringes with a 60 mm stroke length.
Hamilton TLL XL Style Plunger

XB-Style Plunger

This plunger features a stop and a back bushing. The back bushing increases the plunger tip life, maintains proper plunger alignment and reduces the particles generated by plunger and glass contact.
Hamilton C XP Plunger

Bubble Free Prime Plunger (BFP)

This plunger is used exclusively in syringes for Microlab 600 diluters and dispensers. The plunger features a conical plunger tip that extends through the threaded termination of the syringe into the Microlab 600 valve. This design helps remove air from the system and reduces the priming cycles required.
Hamilton BFP Plunger

Reinforced Plunger

Small volume syringes require extremely small plunger wires. For uses that are prone to bending and breaking these small plungers, it is recommended using a reinforced plunger. These plungers are available in both Microliter and Gastight versions in the 800 and 1800 syringe series respectively.
Hamilton Reinforced Plunger

Several different needle point styles are offered on Hamilton syringe and needle products depending on the application. The correct point style varies depending on the intended application. Below are a few examples of common applications.

Manual GC Injections: Historically a Point Style 2 was required to achieve efficient septum penetration with minimized coring. With Hamilton’s new line of GC septa there is evidence that indicates the Point Style AS will consistently extend septa life by as much as 10-fold.

Manual HPLC Injections: Most manual injection valves are designed to be used with a 22 gauge Point Style 3 needle.

Drawing Sample: Any point style will efficiently draw a sample, but when it is important to get the last drop out of a vial use Point Style 3 or AS to reach all the way to the bottom.

Animal Injections: Animal injections require the sharpest needle possible. The recommended needle point style for this application is a Point Style 4 at a 12° bevel. For applications where injection site depth is critical or the target is small, a more vertical bevel as shown below, such as a 30 or 45°, is suggested.

Hamilton Needle Angle

Bevels for the point style 4 needle may vary from 12-60°.

Note: Syringes and needles manufactured by Hamilton Company are intended for scientific research and laboratory use only and are not intended for human in vivo use.

Point Style 2
Sharp, beveled, curved, non-coring needle point recommended for septum penetration.
Available gauges: 34-10.

Hamilton 2 Point Style

Point Style 3
Blunt needle point for use with HPLC injection valves and for sample pipetting.
Available gauges: 34-10.
Hamilton 3 Point Style

Point Style 3T
Blunt needle point for thin layer chromatography (TLC) applications. Needles are coated with PTFE 3/4" up from the needle tip.
Available gauges: 26s, 26, 22s, 22.
Hamilton 3T Point Style

Point Style 4
Standard 12° beveled needle point is recommended for life science applications.
Available gauges: 34-10. Special point styles such as 30°, 45°, or any other angle are available upon request.
Hamilton 4 Point Style

Point Style 5
Conical needle with side port for penetration of septa, thin-gauged vinyls and plastics without coring.
Available gauges: 26-10.
Hamilton 5 Point Style

Point Style AS
Special conical style needle point used on autosampler syringes the non-coring needle point is recommended for septum penetration.
Available gauges: 26-10.
Hamilton AS Poiint Style

Sterilizing and Disinfecting Guide

	Sterilization		Disinfection

Product	Ethylene Oxide	Autoclave	Chemical¹
Cemented Needle (N) Special Cemented Needle (SN)	Yes	No	Yes, no bleach
Luer Tip Cemented Needle (LTN) Luer Tip Special Cemented Needle (LTSN)	Yes	No	Yes, no bleach
Luer Tip (LT)	Yes	Yes	Yes
Removable Needle, (RN)	Yes	Yes	Yes, no bleach
Knurled Hub (KH)	Yes	Yes	Yes
Fixed Needle (FN)	Yes	No	Yes, no bleach
PTFE Luer Lock (TLL)	Yes	Yes, except 25 mL and larger	Yes
Bubble Free Prime (BFP)	Yes	No	Yes
SampleLock (SL)	Yes	No	Yes, no bleach
ChemSeal (C) ChemSeal with Stop (CX)	Yes	Yes	Yes
Carbon Analyzer (CA)	Yes	No	Yes, no bleach
Diluter (D) Diluter with Stop (DX)	Yes	Yes, except 25 mL and larger	Yes
AccuDil (AD) Diluter AccuDil (DAD)	Yes	No	Yes
Constant Rate (CR)	Yes	No	Yes, no bleach
Removable Needles (RN) (18-26s gauge)	Yes	Yes	Yes
Removable Needles (RN) (27-34 gauge)	Yes	Yes	Yes
Metal Hub Needles (N)	Yes	Yes	Yes
Kel-F Hub Needles (KF)	Yes	Yes	Yes
PTFE Tubing	Yes	Yes	Yes
Kel-F Fittings	Yes	Yes	Yes

Note: For syringes that can be autoclaved, they need to be disassembled prior to autoclaving.
¹Recommended disinfecting chemicals are Microcide SQ ® (p/n 3995-01), 10% bleach, acetone or ethanol.

7000 Series Syringes

		Outer Diameter Nominal		Inner Diameter Theoretical Bore
Model	Volume (µL)	mm	Inches	mm	Inches
7000.5 (32 gauge)	0.5	7.8	0.308	0.104	0.0041
7000.5 (25 gauge)	0.5	7.8	0.308	0.104	0.0041
7001 (25 gauge)	1.0	7.8	0.308	0.145	0.0057
7101 (22 gauge)	1.0	7.8	0.308	0.145	0.0057
7002 (25 gauge)	2.0	7.8	0.308	0.206	0.0081
7102 (23 gauge)	2.0	7.8	0.308	0.206	0.0081
7105 (24 gauge)	5.0	7.8	0.308	0.325	0.0128

Note: The plunger of a 7000 series syringe uses a tungsten wire that travels through the syringe to the tip of the needle. The seal is created by a PTFE ferrule within the knurled hub termination. The I.D. of the needle is honed to fit the tungsten wire. The displacement calculation is based on the O.D. of the plunger wire which is displayed as Theoretical Bore above. The O.D. is the outside diameter of the glass barrel.

700 Series Syringes

		Outer Diameter Nominal		Inner Diameter Theoretical Bore
Model	Volume (µL)	mm	Inches	mm	Inches
75	5	6.6	0.260	0.34	0.014
701	10	6.6	0.260	0.49	0.019
702	25	7.7	0.305	0.73	0.029
705	50	7.7	0.305	1.03	0.041
710	100	7.7	0.305	1.40	0.061
725	250	7.7	0.305	2.30	0.091
750	500	7.7	0.305	3.26	0.135

Volumes are based on 60 mm scale length except models 75 and 701 which are based on 54.1 mm scale length. O.D. dimensions are nominal and typical, I.D. dimensions are theoretical.

1700 Series Syringes

		Outer Diameter Nominal		Inner Diameter Theoretical Bore
Model	Volume (µL)	mm	Inches	mm	Inches
1701	10	6.6	0.260	0.46	0.018
1702	25	7.7	0.305	0.73	0.029
1705	50	7.7	0.305	1.03	0.041
1710	100	7.7	0.305	1.40	0.057
1725	250	7.7	0.305	2.30	0.091
1750	500	7.7	0.305	3.26	0.128

1000 Series Syringes

		Outer Diameter Nominal		Inner Diameter Theoretical Bore
Model	Volume (mL)	mm	Inches	mm	Inches
1001	1.0	9.0	0.355	4.61	0.181
1001.25	1.25	8.6	0.329	5.15	0.203
1002 (thin wall)	2.5	9.7	0.375	7.29	0.287
1002 (thick wall)	2.5	10.3	0.407	7.29	0.287
1005	5.0	13.5	0.530	10.30	0.406
1010	10.0	17.7	0.695	14.57	0.574
1025	25.0	27.1	1.067	23.03	0.907
1050	50.0	36.9	1.452	32.54	1.282
1100	100.0	36.9	1.452	32.54	1.282

Volumes are based on 60 mm scale length except models 1100 which are based on 120 mm scale length. O.D. dimensions are nominal and typical, I.D. dimensions are theoretical.

Super Syringes

	Outer Diameter Nominal		Inner Diameter Theoretical Bore
Model	mm	Inches	mm	Inches
S0500	75	2.95	62	2.44
S1000	100	3.95	87	3.44
S1500	100	3.95	87	3.44
S2000	100	3.95	87	3.44

7000 Series Modified Microliter Syringes

	0.5 µL	1 µL	2 µL	5 µL
	7000.5	7001 7101	7002 7102	7105
Scale length, mm	60	60	60	60
Total scale divisions	100	100	100	100
Major graduations, µL	0.05	0.1	0.2	0.5
Minor graduations, µL	0.01	0.02	0.04	0.10
Sub-minor graduations, µL	0.005	0.01	0.02	0.05

600, 700, 800 & 900 Series Microliter Syringes

	2.5 µL	5 µL	5 µL	10 µL	25 µL	50 µL	100 µL	250 µL	500 µL
	62	65	75 85 95	701 801 901	702 802	705 805	710 810	725 825	750
Scale length, mm	27	27	54	54	60	60	60	60	60
Total scale divisions	50	50	100	100	100	100	100	100	100
Major graduations, µL	0.5	1.0	0.5	1.0	2.5	5.0	10	25	50
Minor graduations, µL	0.1	0.2	0.1	0.2	0.5	1.0	2.0	5.0	10
Sub-minor graduations, µL	0.05	0.1	0.05	0.1	0.25	0.5	1.0	2.5	5.0

1700 & 1800 Series Gastight Syringes

	10 µL	25 µL	50 µL	100 µl	250 µL	500 µL
	1701 1801	1702 1802	1705 1805	1710 1810	1725 1825	1750
Scale length, mm	60	60	60	60	60	60
Total scale divisions	100	100	100	100	100	100
Major graduations, µL	1.0	2.5	5.0	10	25	50
Minor graduations, µL	0.2	0.5	1.0	2.0	5.0	10
Sub-minor graduations, µL	0.1	0.25	0.5	1.0	2.5	5.0

1000 Series Gastight Syringes

	1 mL	1.25 mL	2.5 mL	5 mL	10 mL	25 mL	50 mL	100 mL
	1001	1001.25	1002	1005	1010	1025	1050	1100
Scale length, mm	60	60	60	60	60	60	60	120
Total scale divisions	100	100	100	100	100	100	100	200
Major graduations, mL	0.1	0.125	0.25	0.5	1.0	2.5	5.0	5.0
Minor graduations, mL	0.02	0.025	0.05	0.1	0.2	0.5	1.0	1.0
Sub-minor graduations, mL	0.01	0.0125	0.025	0.05	0.1	0.25	0.5	0.5

Super Syringes

	500 mL	1000 mL	1500 mL	2000 mL
	S0500	S1000	S1500	S2000
Scale length, mm	166	167	250	334
Total scale divisions	25	50	75	100
Major graduations, mL	100	100	100	100
Minor graduations, mL	20	20	20	20



Hamilton Robotics Headquarters for North America, South America, Australia & Pacific Rim	Hamilton Robotics Headquarters for Europe, Middle East, Africa & Asia

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Hamilton Company Headquarters for North America, South America, Australia & Pacific Rim	Hamilton Company Headquarters for Europe, Middle East, Africa & Asia

4970 Energy Way Reno, NV 89502 U.S.A	DJ691, nr. Km 8+390m dreapta 307210 Giarmata, jud. Timis Romania

Toll-free Phone: 1-800-648-5950 Phone: +1-775-858-3000 Fax: +1-775-856-7259	Phone: +40 356 635 050 Fax: +40 356 635 060

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	Hamilton Company Headquarters for North America, South America, Australia & Pacific Rim	Hamilton Company Headquarters for Europe, Middle East, Africa & Asia

	4970 Energy Way Reno, NV 89502 U.S.A	DJ691, nr. Km 8+390m dreapta 307210 Giarmata, jud. Timis Romania

	Toll-free Phone: 1-800-648-5950 Phone: +1-775-858-3000 Fax: +1-775-856-7259	Phone: +40 356 635 050 Fax: +40 356 635 060

	Submit an Inquiry


Hamilton Company Headquarters for North America, South America, Australia & Pacific Rim	Hamilton Company Headquarters for Europe, Middle East, Africa & Asia

4970 Energy Way Reno, NV 89502 U.S.A	Via Crusch 8 CH-7402 Bonaduz, GR, Switzerland

Toll-free Phone: 1-800-648-5950 Phone: +1-775-858-3000 Fax: +1-775-856-7259	Phone: +41 58 610 10 10 Fax: +41 58 610 00 10

Find Your Local Representative		Submit an Inquiry

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