Sample recovery is vital to sample purification. Problems arise when labile samples become irreversibly bound to the silanol groups present on C8 and C18 HPLC columns. Since Hamilton polymers are made entirely of poly styrene-divinylbenzene, there are no silanol groups to cause sample loss. Recovery and quantitation of labile and reactive samples is enhanced. The purification of protected oligonucleotides demonstrates the enhanced recovery of polymer supports. While approximately 50–80% of an oligonucleotide is recovered on a C18 column, the equivalent PRP-1 column recovers 95% or greater of the same sample.
Unlike silica-based C8 or C18 columns, PRP-1 has no stationary phase coating. The integral reversed-phase characteristics of the PRP-1 column eliminate the need for special coating techniques. Since there is no stationary phase to hydrolyze, the column maintains its performance characteristics longer than many C8 or C18 columns.
Organic compounds: small molecule (< 2,000 mw), pharmaceuticals, steroids, nucleic acids, vitamins, herbicides
Examples of analytes that can be separated on PRP-1 columns:
- Polycyclic aromatic hydrocarbons (PAH)
- Ionizable organic compounds
- Peptide fragments