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PRP-X300 Columns

Ion exclusion for organic acids and alcohols

Hamilton PRP-X300 columns offer an easy, rapid way to separate closely related alcohols and organic acids. The sulfonated poly(styrene-divinylbenzene) support separates samples via a mixed mode mechanism. Separation on the PRP-X300 is accomplished by three modes:

  • Hydrogen Bonding—The attraction and retention of sample compounds by the
    negatively charged sulfonate group.
  • Reversed-Phase—The interaction and retention of the sample compounds by the non-polar polymeric support.
  • Ion Exclusion—The process in which ionized samples are excluded from the pores of the support and elute first, while the weakly ionized and nonionic compounds elute later.