Hamilton's PRP-3 (12-20 µm 300 Å 21.2 x 250 mm) columns are based on the PRP-1 with a larger pore size for protein separation. This column resin has no silanol groups, so irreversible protein adsorption is not a concern, giving analytes such as proteins a recovery of greater than 90%.
Product Specifications
| Separation Mode |
Separation Mode
Reversed-Phase
|
|---|---|
| Stationary Phase |
Stationary Phase
PRP-3
|
| Format |
Format
HPLC Column
|
| Hardware Inner Diameter |
Hardware Inner Diameter
21.2 mm
|
| Hardware Length |
Hardware Length
250 mm
|
| Particle Size |
Particle Size
12-20 µm
|
| Packing Material Type |
Packing Material Type
PSDVB
|
| Pore Size |
Pore Size
300 Å
|
| Particle Surface Area (m2/g) |
Particle Surface Area (m2/g)
48
|
| Pore Volume (cm3/g) |
Pore Volume (cm3/g)
0.20
|
| Hardware Material |
Hardware Material
Stainless Steel
|
| Max Pressure |
Max Pressure
5,000 psi
|
| Mobile Phase Limits |
Mobile Phase Limits
pH 1-13, 0-100% aqueous, organic modifier
|
| Buffer Strength |
Buffer Strength
0.0-0.5N
|
| Temp Limits |
Temp Limits
5-85°C
|
| Restoration |
Restoration
Run a gradient of 100% water to 100% acetonitrile. Repeat three times
|
| Compound Classification |
Compound Classification
Peptides/Proteins
|
| UOM |
UOM
1 EACH
|