Viable Cell Density Sensors
Incyte Viable Cell Density Sensors - More Data, More Control, More Possibilities
Continuous in-line, or in-situ, data on viable cell density allows for real-time process control. With off-line data, decisions can only be made at the time of sample analysis, resulting in imprecise timing or missing events entirely.
Permittivity is quickly becoming the industry standard for the measurement of viable cell volume because of its robust real-time data. It correlates well to off-line cell counting in the crucial exponential phase without the risk of counting errors. When paired with ArcAir Data Modeling software, this correlation can also be made throughout the process. Incyte Arc sensors can be used to increase process quality across many cell lines, process types, and scales.
Incyte SU (Single-Use)
Incyte Arc
Incyte Viable Cell Density Sensors In Action
From increasing yield to shortening process time, the real power of permittivity is in your hands. As shown in the examples below, ask yourself what you could do with continuous process data.
Process Understanding
Expand your PAT capabilities with permittivity and gain process insights such as cell physiology changes through the use of multi-frequency scan.
Automated Control
Precisely time key process events such as feeding, induction, and harvest for lower production cost, increased yield, and enhanced product quality.
Consistent Quality
Compare permittivity data to ensure batch-to-batch and scale-up reproducibility.
Continuous Process Improvement
Gather on-line viable cell density data every run to enable continuous optimization.
Process Optimization
Real-time data means never missing a key process event. Use this data to develop enhanced process decisions for increased efficiency and prolonged productivity. Alternatively, use ArcAir Data Modeling software for real-time understanding of legacy off-line counts.
Viable Cell Density Soft Sensor
AADM calculates real time viable cell density that correlates to offline measurement and closes all gaps between the measuring points.
The Power of Permittivity for VCD
Permittivity measurements are the most reliable method of monitoring viable cell density (VCD). This measurement is immediately affected by changes in viable cell density and can be used to time process-specific actions for maximum yield. Permittivity can also be used to detect changes in cell physiology and is the most immediate method for determining the beginning of the cell death phase.
Continuous Data
Incyte Arc sensors provide a real-time measurement of viable cell density with continuous permittivity data. Off-line samples only provide limited, error-prone data points, so process events may be missed. Real-time permittivity reveals changes in cell physiology, so advanced process control can be automatically triggered for process optimization.
Available Tools for VCD Sensors
Off-line Totaland Viable Cell Density |
Viable CellBio-Volume with Incyte |
VCD SoftSensor with Incyte Arc |
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| Benefits and Attributes | Discrete Data Points Sampling Required Contamination Risk Enables Process Control Real-Time Decisions Continuous Measurement Reduce Sampling |
✚ ✚ High ✖ ✖ ✖ ✖ |
✖ ✖ Low ✚ ✚ ✚ ✚ |
✖ ✖ Low ✚ ✚ ✚ ✚ |
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*Sampling required during initial modeling and model maintenance
Will Hamilton Cell Density Sensors Work with My Process?
Review Hamilton's Cell Density Applications Ebook
Hamilton cell density sensors are compatible with many types of cell lines and microbial strains, process types, and industries. Read more below or download our Cell Density Applications eBook to see real data with different cell lines and process types.
Start a Conversation
After starting a conversation with a Hamilton's team member, you will have access to an applications specialist to discuss the potential of Hamilton Incyte Arc sensors in your bioprocess.
Leverage Hamilton Expertise
Utilize Hamilton’s Application Team and proven evaluation process for rapid implementation and robust product evaluation.
Hamilton Application Team Support
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Dencytee Setup
Hamilton evaluates your application to recommend quick-start measurement settings.
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Collect Data
Follow Hamilton’s standard protocol to ensure robust and reliable data sets.
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Analyze Results
Hamilton’s Application Team can support your analysis and generate a comprehensive report with useful insights.
Sensors for Single-Use
Hamilton has adapted its trusted sensor technology to single-use environments, including solutions for pH, dissolved oxygen, conductivity, and viable cell density. Now available for bag manufacturers and other integrators.
Accessories
ArcAir Software
Arc Wi Wireless Adapters
Arc USB Power Cables
ArcAir Data Modeling Software
Specific Applications
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App Note - Human Platelet Protein Production from Human Cell Culture using an Advanced Bioreactor
Online viable cell density monitoring in a platelet bioprocess production. -
App Note - Correlation between Capacitance Signal and Viable Cell Density of Insect Cells
Wild type Sf-9 cell-line vs. polyclonal stable Sf-9 cell line -
App Note - Continuous Cyanobacteria - Limnospora (Spirulina) Monitoring in the MELiSSA Loop using Dencytee
Drift free long term biomass monitoring for better understanding of process dynamics
Resources
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Catalog - Process Analytics
Download the Process Analytics sensor catalog to learn about the complete offering of Hamilton process sensors and accessories. -
Quick Guide - Validation / Calibration Incyte Sensors (Pre-amp Version)
Quick Guide for Validation / Calibration Incyte (Pre-amp Version) -
Quick Guide - Arc Module Incyte-P SU
Quick Guide for Arc Module Incyte-P SU
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How often do Incyte sensors (pre-amp version) need to be calibrated?
Hamilton recommends once per year with a validation at 6 months. -
How does Incyte measure viable cell density?
Incyte is a capacitance-based sensor responsive only to living cells. -
What effect will bubbles have on the Incyte sensor measurement?
Gas bubbles have two main impacts: (1) Decreased signal intensity since the sensor cannot measure in air. (2) Increased background noise.
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Cell Density Measurement Guidelines
Process Analytical Technology (PAT) is crucial in any biopharma application. One Key Process Indicator critical to PAT implementation is how many cells are present (also known as cell density). -
Incyte Cell Density Measurement Principles
Incyte enables the dual frequency measurement mode, or a frequency scanning. Learn about how Hamilton Incyte cell density sensors can continuously measure viable cells. -
Incyte Arc PCS Connections for Existing Cell Density Users
This article reviews changes in the sensors connection strategy between the older pre-amp version of Incyte Cell Density products and the newer Incyte Arc products.
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