White Paper: Measurement Challenges with Optical Dissolved Oxygen Sensors
Are you missing sources of measurement error?
Measurement of dissolved oxygen (DO) has long been known as a critical control parameter for optimizing cell growth in bioprocesses. Much has been written about different strategies for proper DO control. However, little information has been publicly available regarding optimization of the actual measurement accuracy and reducing common sources of process-related error. This paper aims to divulge these details for the first time. Specific consideration will be given to optical dissolved oxygen measurement which has become the predominant measurement technology in bioprocesses.
Common Sources of DO Measurement Error
Calibration Errors
Proper calibration plays an important role in maximizing measurement accuracy.
Photobleaching
Current oDO sensors may exhibit error due to changes in the luminophore from photobleaching or chemical attack.
Cumulative Effects of SIP/CIP/Photobleaching
The net error accumulation of these factors is significant and can lead to frequent post-run verification deviations in GMP/FDA environments.
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