For first time sensor use, check functionality prior to insertion into the bioreactor. Connect the sensor to ArcAir and ensure sensor communication in ArcAir. Confirm there are no warnings or errors present that may indicate damage during shipping.
Between process runs, inspect the sensor tip and mounting o-ring. Ensure that the window is scratch-free and clear of debris or biofilm. Verify that the o-ring is intact and properly located below the PG13.5 mounting thread.
When positioning Dencytee Arc in the bioreactor, ensure that the sensor is fully submerged into a region that allows consistent solution movement through the sensing gap while preventing bubble entrapment due to sparging and agitation. Choose a sensor with an appropriate a-length to effectively measure a region of homogeneous media, unaffected by cavitation or low-mixing. Suggested positioning requires the sensing gap face away from stirrers and spargers for (A) side and (B) top installations:
Ensure that the sensor is installed in a port that is electrically conductive (<1MΩ) and the sensor shaft and/or VP8 cable shield is grounded. Metal bioreactors can be directly grounded if the tank is grounded itself (left), while glass or plastic bioreactors will require additional grounding at the sensor port (right). If the metal bioreactor is not grounded itself, follow the diagram on the right:
Once the sensor is properly positioned, hand tighten the probe into the bioreactor port. Your Dencytee Arc sensor is now ready for use.
If using a Wi Adapter, do NOT use it as a leverage point for installation/removal of the sensor! Do not use wrenches to install or remove sensors!