DATE: APRIL 2019
The University of Melbourne | Physiology

Garron Dodd
Senior Lecturer

We will use these Hamilton products to investigate how the brain controls metabolic diseases such as obesity and type-2 diabetes. If successful we will use this diverse range of Hamilton Syringes to; 1) Stereotaxically administer highly precise amount of virus into specific regions of the rodent brain using syringes 65456-01 and 65457-01. These products will afford us an unparalleled accuracy in how we can genetically edit the brain, an approach that would be far superior to using glass micropipettes. This will set a new standard in how agents (virus, drugs etc) are accurately delivered to discrete regions of the brain with high reproducibility. 2) Load protein and RNA gels with much higher accuracy than that of a standard pipette. Routinely people use standard pipettes to loads RNA and protein gels. Here we have developed a method to extract low amounts of protein and RNA from very small punches of the rodent and human brain. To accurately analyse this we require syringes 80360 and 80465 to accurately load these precious samples with high reproducibility. This will set a new standard by which precious samples with low amounts of starting material are analysed by gel separation.

These products will allow us to deliver a virus capable of deleting the insulin receptor in small regions of the hypothalamus that are known to become defective in obesity and T2D. Current practice using glass micropipettes results in inaccurate virus delivery and high variation between subjects. Using syringes 65456-01 and 65457-01we will be able to overcome these past limitations to gain valuable insight into how to target the brain to treat metabolic disease. Furthermore, we can then explore how these small regions of the hypothalamus become defective in metabolic disease by microdissecting out these brain regions and investigating the transcriptome and proteome. Using syringes 80360 and 80465 we will be able to load homogenates of the brain with high precision and reproducibility, a feat previously unattainable using standard pippetes. As well as improving research outcomes, using these syringes we will be able to treat multiple cohorts of students (>30 per year NEURO 3009 - Neurophsyiology) how to conduct these cutting edge neuroscience techniques.

It is our lab's mission to understand how the brain coordinates metabolic disease such as obesity and type 2 diabetes and how to define novel therapeutics. To do this we utilise the latest technology and international collaboration alongside a strong teaching ethos to generate compelling research outcomes with building the next generation of scientists. What really sets us apart is that we use Hamilton syringes in nearly every aspect of our workflow from molecular biology, proteomics to in vivo neuroscience.

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