How Do I Connect a Stainless Steel Guard Column to My HPLC Column?
Locate the tubing in the HPLC that connects the injection valve to the inlet of the HPLC column. This tube will be made of stainless steel or PEEK, and is referred to as the column inlet tube or column inlet line.
If the end of the column inlet tube that goes into the HPLC column is bare, with no nut and ferrule, place a stainless steel male nut (10-32 threads) for 1/16 OD tubing over the tubing at the end where the tube goes into the inlet of the column with the threads facing toward the inlet of the HPLC column, and place a stainless steel compression ferrule over the column inlet tube with the tapered end facing toward the open end of the tube.
To connect the stainless steel guard column to the HPLC column, use a short piece of 1/16" OD stainless steel tubing (~ 50 mm in length) and place a stainless steel male nut (10-32 threads) for 1/16 OD tubing and ferrule on both ends of the tubing. This short piece of tubing will connect the guard column to the HPLC column.
Using a 3/8" open end wrench to hold the the guard column end cap, tighten the stainless steel nut/ferrule on the connecting tubing into the threaded end fitting of the guard column until the connection is tight and the tubing cannot be pulled loose. Repeat this step to connect the other end of the connecting tubing to the inlet of the HPLC column.