PRP-X300 Columns
Hamilton PRP-X300 columns offer an easy, rapid way to separate closely related alcohols and organic acids. The sulfonated poly(styrene-divinylbenzene) support separates samples via a mixed mode mechanism. Separation on the PRP-X300 is accomplished by three modes:
- Hydrogen Bonding—The attraction and retention of sample compounds by the negatively charged sulfonate group.
- Reversed-Phase—The interaction and retention of the sample compounds by the non-polar polymeric support.
- Ion Exclusion—The process in which ionized samples are excluded from the pores of the support and elute first, while the weakly ionized and non-ionic compounds elute later.
Resources
Hamilton's knowledge base provides a range of explanatory articles, frequently asked questions, and document downloads.
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USP "L" Column Listings
Explore Hamilton’s qualifying USP “L” columns. -
Choosing Column Specifications
The sample amount needed to be purified or separated for analysis will dictate which scale of chromatography is needed. In general, the scales of chromatography are microbore, analytical, semi-preparative, and preparative. Nanoscale and microscale are smaller scale ranges but are not covered in Hamilton’s product offerings. READ MORE
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