Nucleotides on 150 x 4.1 mm PRP-X100, P/N 79434
Application #74
HPLC Column
Chromatographic Conditions
Injection Volume | 20 µL |
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Detection | UV at 254 nm |
Column Dimensions | 150 x 4.1 mm |
Eluent A | 25 mM Citric Acid |
Eluent A pH | 5.4 |
Eluent B | 17:3 25 mM Citric Acid : Acetonitrile |
Eluent B pH | 5.4 |
Flow Rate | 2.0 mL/min |
Compounds
1 | Cytidine 5'-Monophosphate (CMP) |
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2 | Uridine 5'-Monophosphate (UMP) |
3 | Guanosine 5'-monophosphate (GMP) |
4 | Thymidine 5'-monophosphate (TMP) |
5 | Adenosine 5'-monophosphate (AMP) |