Case Studies
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A comprehensive investigation of purifying oligonucleotides.
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With the growing utility of the K vitamin series of molecules, a need appears relevant for both food labeling of constituents and for research based separation of the natural and synthetic compounds of vitamin K.
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In this study of an essential oil, we have developed an HPLC method for the separation of 6 well-known markers for the determination of purity of Ylang-Ylang oil.
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Soy beans can contain a fungal metabolite and mycotoxin, Zearalenone (ZEA), which cause an increase in the proliferation of certain tumor cells
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In this study, 9 structurally-diverse, strongly basic drug compounds are resolved in less than 2 minutes using a high pH mobile phase and a fast acetonitrile gradient at 80°C.
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Rapid separations under alkalin pH conditions.
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A reversed-phase HPLC method was developed for separation of five steroid hormones with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone) on a Hamilton PRP-C18 HPLC column.
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At any pH
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In some cases it has been shown that allergies can arise from some of the individual components of the essential oil Balsam of Peru. Read our app note detailing an HPLC method that separates five components known to have allergenic properties
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The separation of metabolites from benzodiazepine use is examined using reversed-phase chromatography.
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Using ion-pair reversed-phase HPLC to separate nucleotides ATP, ADP and AMP from like-phosphorylated guanosine metabolic products from tissue extracts.
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Hamilton PRP-1 polystyrene-divinylbenzene RP-column has been compared to two C18 silica stationary phases for the separation of sibutramine, an antiobesity drug, using paroxetine as an internal standard.
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Resolution of arsenic compounds is achieved by anion exchange chromatography on a Hamilton PRP-X100 column.
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Hamilton PRP-X100 and PRP-X110 are the perfect solution for anion analysis in water or environmental samples.
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Isolate toxic Cr(VI) from its essential nutrient Cr(III)
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Two anion exchange HPLC columns for a wide range of applications.
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Establishing an accurate standard method of analysis for the determination of total glucose content in parenteral nutrition solutions.
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With health, safety, and edibles dosing as the primary motivation, Hamilton Company developed an HPLC method that isolates eight major cannabinoids. The HxSil C18 (3 µm) column provides an accurate, cost effective, and robust solution that can be used in any HPLC system.
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In this application note we have developed an HPLC method that isolates five different aflatoxins in less than 6 minutes.