How do I replace CO-RE II stop discs and O-rings?

O-Ring Replacement

Requirements

Purchased from Hamilton:

• P/N 6607080-01 CO-RE II O-ring 2 Pack for 2 channels or P/N 6607080-02 CO-RE II O-ring 10 Pack for 2 Channels + 8 Channel Multi-probe head

• Tips

You will need to supply:

• Protective gloves (and other appropriate PPE for your lab)

• Clean, lint-free towels

• Deionized water

The following procedure describes the steps for O-ring replacement in detail.

1. Make sure to wear appropriate PPE for the lab. At minimum, gloves are required for stop disc and O-ring replacement.
2. Turn on the Prep if it is not already on. Sign in if required.
3. Tap the settings button with the gear icon at the top-right corner of the home page.
4. Run the appropriate “Replace stop discs and O-rings” protocol. Each channel has its own routine, as well as the 8-channel MPH.
5. Clear any labware on the deck and close the door. The Prep will move the pipetting arm and tools into position. Open the door again when prompted.
6. Once prompted, select O-rings to be replaced. Select “Next”.
7. Follow onscreen instructions to replace O-rings using included pick tool.
8. Wipe down the new stop disc with a clean, lint-free towel sprayed with deionized water.
9. Repeat steps 4–8 as necessary. All eight O-rings on the multi-probe head must be replaced. The pipetting heads for the channels only have one stop disc each, but they should be replaced at the same time for consistent performance.
10. Close the door when prompted. The Prep will start the calibration procedure for the new stop discs and O-rings. Follow the instructions onscreen to complete calibration.
11. When prompted, open the door and place tips on site 7. If your prep has independent channels only, you will need two tips in positions A1 and B1. Otherwise, you will need a full column of tips in column 1.
12. Specify which tips you loaded onscreen, then tap “Next”.
13. Close the door and wait until calibration is finished. You can remove the tips once calibration is finished.

Stop Disc Replacement

Requirements

Purchased from Hamilton:

• Set of CO-RE II stop discs (2-pack for channels P/N 97660-02; 8-pack for 8 MPH P/N 97660-08)

• CO-RE II stop disc removal tools P/N 6607023-01

• Tips

You will need to supply:

• Protective gloves (and other appropriate PPE for your lab)

• Clean, lint-free towels

• Deionized water

The following procedure describes the steps for stop disc replacement in detail.

NOTE: Requires CO-RE II stop disc tools (P/N 6607023).

1. Make sure to wear appropriate PPE for the lab. At minimum, gloves are required for stop disc and O-ring replacement.
2. Run the appropriate “Replace stop discs and O-rings” protocol.
3. Clear any labware on the deck and close the door. The Prep will move the pipetting arm and tools into position. Open the door again when prompted.
4. Use the 12 cNm torque driver and 2 mm hex bit to unscrew the old stop disk from the pipetting head or multi-probe head, along with the old O-ring and drive ring.
5. Remove rubber sleeve from the stop of CO-RE II stop disc.
6. Make sure the drive ring is properly attached to the new stop disc. The drive ring should sit on top of the stop-disk.
   
   
7. Screw the new stop disc onto the pipetting head or multi-probe head using the 12 cNm torque driver and 2 mm hex bit. The drive ring may need to be adjusted slightly to fit properly in place.
   • WARNING: Do not use any other tools to tighten the new stop disc. Doing so may damage the pipetting head.
8. Wipe down the new stop disc with a clean, lint-free towel sprayed with deionized water.
9. Repeat steps 4–8 as necessary. All eight stop discs on the multi-probe head must be replaced. The pipetting heads for the channels only have one stop disc each, but they should be replaced at the same time for consistent performance.
10. For the multi probe-head, ensure all stop discs are flush.

Close the door when prompted. The Prep will start the calibration procedure for the new stop discs and O-rings. Follow the instructions onscreen to complete calibration.