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The Zyppy™ procedure allows for high-throughput automation and requires no centrifugation or pelleting of cells. The technology features a modified alkaline lysis system that allows for the direct lysis of E. coli. Sample variability is greatly reduced by using the Microlab STAR and the resulting endotoxin-free and high quality DNA is ready for restriction endonuclease digestion, ligation, PCR, transformation, sequencing, etc.