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The BioAnalytica-Genotypos center in Athens chose to couple the Hamilton STARlet robot with the SOPHiA Clinical Exome Solution

(CES) for dealing with the increasing workload they were facing. In fact, over the past decade, the number of samples and

types of analyses processed in the laboratory increased exponentially, leading to a pressure to develop, optimize, and validate

NGS high-throughput assays.

To ensure high-quality results on the new workflow and facilitate its implementation, the automated protocol has been extensively

tested by SOPHiA GENETICS and Hamilton on a smaller representative panel of 128 genes. The validation study ensured

a reliable and flexible automation, which has been efficiently applied on larger gene panels, including CES.