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Media and Buffer Preparation

Media Prep Tank

The tank consists of a stainless steel vessel jacketed for heat transfer with the following associated equipment: an agitator, a sterile vent filter, a sterile liquid filter, a pump depending on type of tank (media transfer pump, buffer solution transfer pump, or a chemical injection pump), and a control and instrumentation system for monitoring and adjustment of the system pressure, temperature, and fluid level and for monitoring pH and conductivity. The system including the interior surface of the vessel, piping, valves, and any surface in potential contact with process streams is designed to be cleaned-in-place (CIP) and sterilized-in-place (SIP). The sterilization is performed to achieve a probability of non-sterility not greater than one in a million.

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