High content screening (HCS) is a method used in drug discovery to identify substances such as small molecules and others that alter the phenotype of a cell. It typically involves monitoring changes in the amount and/or the location of cellular products, such as proteins, or the morphology of the cells, usually using fluorescent dyes or label-free methods.
Typically, cells are incubated with the substance of interest and monitored after a certain period of time. Because of the simultaneous, automated readout of several parameters at once, a large number of data points can be collected per assay. To enable high throughputs with this technique, it is necessary to automate the entire process, including dispensing substances, maintaining the cells during the process and readout. Hamilton can deliver automated solutions integrating high content readers with its liquid handlers.