Advanced cell-based research requires cell isolation techniques in addition to cell culture. Magnetic-based separation can separate cells of almost any type from whole blood, bone marrow or buffy coat. Typically, CD antibodies or antigens are coupled to nanosized paramagnetic or superparamagnetic molecules. When mixed with cells and incubated under certain buffer conditions, the now-conjugated magnetic molecules are exposed to a magnetic field to perform the separation. Positive or negative selection can be performed, depending on the needs of the assay. This technique yields highly purified isolations of cells for a variety of downstream cell-based applications, including flow cytometry, sequencing or sample enrichment.
Hamilton Robotics' automated solutions are adaptable to a variety of magnetic-based cell separation techniques. Instead of using stand-alone automated solutions for magnetic separation, Hamilton Robotics solutions integrate multiple steps in cell-based research. These systems enable more freedom in selecting scientific solutions because many different magnetic cell separation systems can be integrated.