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Mammalian Culture and Maintenance

Mammalian cells

Hamilton’s Automated Cell Culture Systems offers Flexibility & Consistency

Mammalian cells can be cultured and maintained in a variety of ways, including different culture vessels and varying volumes of different media types. Some cell types (for example, stem cells) may also require a basement membrane matrix for growth or even feeder cells and co-culture for differentiation purposes. Mammalian cells also have to be environmentally controlled, continually monitored for growth and fed, passaged, harvested and re-seeded.

To prepare these cells for research and screening, researchers must plan when cells will be grown up, and scheduling them to be ready on a certain date can be extremely challenging.

With Hamilton’s Automated Cell Culture Systems, researchers can better plan and schedule their cell culture scale-up and assay-ready plates. Automating these processes also offers flexibility for different culture parameters and provides process consistency. Our systems are also completely automated: they control the culture environment and perform routine cell processing without user intervention.

Features of Automation


Benefits of Automation

  • Scalable and flexible platforms and software
  • Venus software enables:
    • Coordination of cell culture activities over long periods of time
    • Sample and data tracking
  • Contamination-free workspace and system
  • Automated air displacement pipetting technology allows for contamination-free liquid handling compared to liquid-filled systems
  • Pipetting volume range of 0.5 to 5000 µL
  • Automating cell culture bypasses the major bottlenecks of manual cell maintenance. Benefits include:

    • Process control
      • Cell plating
      • Media exchange
      • Confluence monitoring
      • Passaging
      • Harvesting
      • Re-seeding
    • Multiple maintenance protocols can be combined on one system
    • Reproducible and standardized
    • Prevents cell line cross contamination due to misidentification

Common Workstation Components

  • Microlab STAR 8 Channel System with Plate Gripper and optional Multi-Probe Head
  • CO2 controlled Incubator
  • Confluence Imager
  • HEPA Filtered Hood
  • On-deck Heating and Tilting Modules
  • Media Supply Modules