ThermoFinnigan HS 250, HS 500, & HS 850 Syringes

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                Syringe Type

                Key to selecting the right syringe for your application is to identify your sample type and determine the smallest volume to be dispensed or injected. Hamilton offers two types of syringes, GASTIGHT and MICROLITER, which differ in the design of the plunger.



                GASTIGHT Syringes for Gases and Liquids

                GASTIGHT syringes have a precision-machined PTFE plunger tip which creates a leak-free seal. With the tight fit, the tip essentially wipes the interior of the syringe barrel free of sample. This feature is particularly useful with heterogeneous samples as it reduces the chance that a deposit will occur and cause the plunger to freeze.

                The GASTIGHT series still requires careful and regular cleaning to minimize deposits on the glass which might score the soft PTFE plunger tip and resulting in a leak. Replacement plunger assemblies are available for GASTIGHT syringes. However, a replacement plunger should not be put into a barrel which still contains deposits because the new plunger tip is likely to be scored by the deposit after only a few strokes. It is important to remember that over time, the increased friction created by the tight seal may cause the PTFE tip to wear out and the plunger will have to be replaced.
                  MICROLITER Syringes for Liquids

                MICROLITER syringes incorporate a hand-fitted stainless steel plunger with a finely bored syringe barrel. These syringes are ideal for homogenous samples that are not prone to precipitation or bonding with glass.

                Under the proper conditions, plunger wear is minimal and the life of a MICROLITER syringe is almost unlimited. However, when using heterogeneous solutions with a MICROLITER syringe, the user must be especially diligent about cleaning the syringe after each use. For more information, see our Guide to Maintaining and Using Hamilton Syringes.

                In some cases, even diligent cleaning is not sufficient and the barrel will become soiled. The deposits on the glass will compromise the tight tolerances between the glass and the plunger resulting in a frozen plunger. Plungers for MICROLITER syringes cannot be interchanged or replaced if damaged. For heterogeneous solutions, a GASTIGHT syringe is the best option.

                Volume

                For gas samples and heterogeneous liquids, there are four GASTIGHT syringe series from which to choose. For homogeneous liquid samples, there are five MICROLITER syringe series. Given the variety of syringe series available with MICROLITER and GASTIGHT plungers, an easy way to narrow down the list of series is to use delivery (or dispensing) volume as a discriminating factor. For the most accurate dispenses always choose a syringe that has a nominal volume as close to the dispense volume as possible.

                For accuracy and precision, the smallest dispensing volume for a given syringe should be greater than or equal to 10% of its total capacity. For example, the smallest dispensing volume recommended for a 10 µL syringe is 1 µL. The following Syringe Type to Series Chart shows the volumes that each series is capable of dispensing within 10-100% of the syringes’ nominal volume.

                Syringe Series

                MICROLITER Syringe Reference
                (0.05 µL - 500 µL Delivery Volume)



                600 Series (0.25 µL - 5 µL Delivery Volume)

                The 600 series are robust syringes consisting of two distinct parts. The bottom half of the barrel accurately measures the liquid sample and the top half supports the plunger. In addition, the top section of the plunger is thicker to further reduce the risk of bending the plunger. These syringes require half of the standard stroke length making them ideal for one-handed operation. The plungers and barrels are not interchangeable or replaceable.

                Recommended Use: The 600 series is great for animal injections because one hand is free for manipulating the animal.
                Volumes: 2.5 µL and 5 µL
                Terminations: Removable Needle (RN)
                Accessories: Syringe Guide and Reproducibility Adapter
                700 Series (0.5 µL - 500 µL Delivery Volume)

                The 700 series is the original Hamilton syringe. It was designed to solve the general liquid handling requirements of manufacturing and research laboratories and remains the industry standard. The plungers and barrels are not interchangeable or replaceable.

                Recommended Use: The 700 series is used for manual and automated GC and HPLC injections. Also, it is used for everyday applications that require accurate measuring and dispensing of liquid.
                Volumes: 5 µL, 10 µL, 25 µL, 50 µL, 100 µL, 250 µL, and 500 µL
                Terminations: Cemented Needle (N), Special Cemented Needle (SN), Removable Needle (RN) and Luer Tip (LT)
                Accessories: PB600 Repeating Dispenser, Reproducibility Adapter, Syringe Guide, and Digital Syringe
                800 Series (0.5 µL - 250 µL Delivery Volume)

                The 800 series has the same liquid handling capabilities as the 700 series, but with the addition of an aluminum syringe holder designed to eliminate the possibility of plunger damage. The syringe holder screws onto the glass barrel. A two-piece extended plunger further eliminates the risk of breakage. A friction screw at the top of the barrel can be adjusted to give the user some control over dispensing speed and prevent the accidental removal of the plunger. In addition, the syringe holder can be fitted with different barrel/plunger assemblies to cover a range of volumes.

                Recommended Use: This syringe is intended for applications where the plunger on a 700 series syringe might be bent. Excellent for novice users.
                Volumes: 5 µL, 10 µL, 25 µL, 50 µL, 100 µL, and 250 µL
                Terminations: Cemented Needle (N), Special Cemented Needle (SN), and Removable Needle (RN)
                Accessories: Reproducibility Adapter
                900 Series (0.5 µL - 10 µL Delivery Volume)

                The 900 series has the same liquid handling capabilities as the 800 series but is a more economical version. The 900 series does not have any replaceable parts making the initial cost less than the 800 series.

                Recommended Use: This syringe is perfect when a robust syringe is desired and budget is a consideration.
                Volumes: 5 µL and 10 µL
                Terminations: Cemented Needle (N), Special Cemented Needle (SN), and Removable Needle (RN)
                Accessories: Reproducibility Adapter
                7000 Series (0.05 µL - 5 µL Delivery Volume)

                The 7000 series employs a plunger wire inside the needle to accurately dispense ultra-low volumes. The needle is bored to extremely accurate tolerances to accommodate the plunger wire. With the plunger inside the needle, the standard dead volume inside the needle is eliminated.

                Recommended Use: The 7000 series is used for manual and automated GC and HPLC injections. Also, it is used for everyday applications that require ultra-small measurements of liquid.
                Volumes: 0.5 µL, 1 µL, 2 µL, and 5 µL
                Terminations: Knurled Hub (KH)
                Accessories: Reproducibility Adapter, Syringe Guide,Digital Syringe, and Syringe Cleaner


                GASTIGHT Syringe Reference
                (1 µL - 2000 mL Delivery Volume)



                1000 Series (100 µL - 100 mL Delivery Volume)

                The 1000 series is a mid-volume solution for all liquid and gas handling needs.

                Recommended Use: The 1000 series is ideal for manual and automated HPLC injections. This syringe is commonly used in syringe pumps and other liquid handling instrumentation
                Volumes: 1 mL, 1.25 mL, 2.5 mL, 5 mL, 10 mL, 25 mL, 50 mL, and 100 mL
                Terminations: Luer Tip Cemented Needle (LTN), Luer Tip Special Cemented Needle (LTSN), Luer Tip (LT), Removable Needle (RN), SampleLock (SL), and PTFE Luer Lock (TLL)
                Accessories: PB600 Repeating Dispenser for volumes (1 to 2.5 mL)
                1700 Series (1 µL - 500 µL Delivery Volume)

                This is the GASTIGHT version of the original Hamilton 700 series syringe. It was designed to meet the low volume gas or liquid handling needs of research and manufacturing laboratories.

                Recommended Use: The 1700 series is excellent for manual and automated GC and HPLC injections. This syringe is commonly used in syringe pumps and other liquid handling instrumentation. Volumes: 10 µL, 25 µL, 50 µL, 100 µL, 250 µL, and 500 µL
                Terminations: Cemented Needle (N), Special Cemented Needle (SN), Luer Tip Cemented Needle (LTN), Luer Tip Special Cemented Needle (LTSN), Luer Tip (LT), Removable Needle (RN), SampleLock (SL), and PTFE Luer Lock (TLL)
                Accessories: PB600 Repeating Dispenser, Reproducibility Adapter, Syringe Guide, and Digital Syringe
                1800 Series (1 µL - 250 µL Delivery Volume)

                The 1800 series was designed to eliminate the possibility of plunger damage. It has the same liquid handling capabilities as the 1700 series but the extended plunger eliminates breakage and allows the user some control over the dispense speed.

                Recommended Use: This syringe is intended for applications where the plunger on a 1700 series syringe might be bent.
                Volumes: 10 µL, 25 µL, 50 µL, 100 µL, and 250 µL
                Terminations: Cemented Needle (N) and Removable Needle (RN)
                Accessories: Reproducibility Adapter
                Super Syringe (50 mL - 2L Delivery Volume)

                Super Syringes were designed primarily for air sampling, preparing gas standards, calibrating reservoirs, and pneumographs. The Super Syringe is the only Hamilton syringe with an acrylic barrel, which makes this syringe slightly less chemically resistant.

                Recommended Use: This syringe is intended for sampling or transfer of gases.
                Volumes: 0.5L, 1L, 1.5L, and 2L
                Terminations: PTFE Luer Lock (TLL) and Tracheal Adapter (accepts 5/8 inch I.D. flexible tubing)
                Accessories: None

                Termination

                Terminations are located at the end of the syringe barrel and function as the interface between the syringe and its mating connection such as the needle. Terminations are offered in a number of different needle and connection configurations to accommodate a broad range of applications.

                Below is a listing of the most popular syringe terminations.

                N, Cemented Needle
                For low volume syringes the needles are cemented into the glass syringe barrel at a point corresponding to the zero graduation mark. With this termination, dead volume is limited to the internal volume of the needle. Not autoclavable. Needle gauge is determined by the syringe volume and are not user-selectable.
                SN, Special Cemented Needle
                For low volume syringes the special needle terminations are the same as the Cemented Needle terminations except they allow for a variety of user-defined gauges, lengths, and point styles to be attached.
                LTN, Luer Tip Cemented Needle
                For mid volume syringes the needles are cemented into the glass syringe barrel at a point corresponding to the zero graduation mark. With this termination, dead volume is limited to the internal volume of the needle. Not autoclavable. Needle gauge is determined by the syringe volume and are not user-selectable.
                LTSN, Luer Tip Special Cemented Needle
                For mid volume syringes the special needle terminations are the same as the Luer Tip Cemented Needle terminations except they allow for a variety of user-defined gauges, lengths, and point styles to be attached.
                LT, Luer Tip
                The needles are removable and fit over a ground glass hub which is tapered in the shape of a male luer. The LT termination will accept most hypodermic needles but was designed specifically for use with Hamilton Kel-F needles. This termination increases the dead volume in the syringe, which may not be appropriate for some applications. Autoclavable when disassembled.
                RN, Removable Needle
                The needles are removable and are a Hamilton-specific design. The design allows the needles to seat precisely at the zero graduation mark of the syringe. Users can select the needle gauge, length, and point style to optimize the syringe for custom applications. Additionally, this termination allows for a removable needle without increasing the dead volume of the syringe and is ideal when there is a risk of the needle clogging. Autoclavable when disassembled. Repeated autoclaving will shorten syringe life.
                KH, Knurled Hub
                The knurled hub is used exclusively on 7000 Series syringes. The hub handles up to 6000 psig maximum injection pressure. The needle is removable but with a limited number of gauges available because the plunger is fitted inside the needle. Autoclavable when disassembled. Repeated autoclaving will shorten syringe life.
                TLL, PTFE® Luer Lock
                This termination has a PTFE, male Luer taper with nickelplated brass locking hub for use with Kel-F needles, metal hub needles, and universal connectors. Also, the TLL is used with Hamilton diluters/dispensers, OEM applications, and manual operations. Autoclavable when disassembled, except on 25 mL and greater syringes. Repeated autoclaving will shorten syringe life.
                SL, SampleLock™
                The sample lock incorporates an On/Off syringe valve with RN needle. This termination is used for headspace, environmental sample collection and storage, pre-pressurization of gaseous samples for GC analysis, and sample spiking. Not autoclavable.

                Gauge

                When selecting a needle gauge it is important to keep in mind the volume of the syringe and the dead volume of the needle. For example, it will be very difficult to prime a 10 µL syringe if the dead volume in the needle is greater than 10 µL. Refer to the gauge index to choose a needle gauge with an appropriate µL/inch before selecting a needle. Select the minimum length that allows you to carry out your application comfortably.

                Note: The ‘s’ on a 22s needle represents a smaller I.D. (inner diameter) for the needle and a thicker needle wall for better durability. For example, a 26 gauge needle has an O.D. (outer diameter) of 0.46 mm and an I.D. of 0.26 mm while the 26s gauge needle has an O.D. of 0.47 mm and an I.D. of 0.13 mm. The 26s has half the I.D. of the 26 gauge needle. Also, the difference in the wall thickness nearly doubles with 26s gauge having a thickness of 0.18 mm while the 26 gauge is only 0.10 mm.

                Length

                The standard length needle for a Hamilton syringe is 2 inches or 51 mm. In some cases other lengths are available but if a custom length is desired this can be ordered through the Custom Needle Program. Simply select the "Custom Needle" option or the "No Needle Included" option from the length menu on the filter tool. When you select a product that has a custom needle you will have to specify the desired length during the ordering process. When you select the no needle option you will have to purchase a compatible custom needle six pack along with the syringe order.

                Point Style

                Several different needle point styles are offered on Hamilton syringe and needle products depending on the application. The correct point style varies depending on the intended application. Below are a few examples of common applications.

                Animal Injections: Point Style 4 with a 45º angle allows for the most accurate targeting of a specific biological structure.

                Manual HPLC Injections: Most manual injection valves are designed to be used with a 22 gauge Point Style 3 needle.

                Manual GC Injections: Historically a Point Style 2 was required to achieve efficient septum penetration with minimized coring. With Hamilton’s new line of GC septa there is evidence that indicates the Point Style AS will consistently extend septa life by as much as 10-fold.

                Drawing Sample: Any point style will efficiently draw a sample, but when it is important to get the last drop out of a vial use Point Style 3 or AS to reach all the way to the bottom.

                Note: Syringes and needles manufactured by Hamilton Company are intended for scientific research and laboratory use only and are not intended for human in vivo use.


                Point Style 2
                Sharp, beveled, curved, non-coring needle point recommended for septum penetration.
                Available gauges: 33-10.






                Point Style 3
                Blunt needle point for use with HPLC injection valves and for sample pipetting.
                Available gauges: 33-10.






                Point Style 4
                Standard 12° beveled needle point is recommended for life science applications.
                Available gauges: 33-10. Special point styles such as 30°, 45°, or any other angle are available upon request.






                Point Style 5
                Conical needle with side port for penetration of septa, thin-gauged vinyls and plastics without coring.
                Available gauges: 26-10.






                Point Style AS
                Special conical style needle point used on autosampler syringes the non-coring needle point is recommended for septum penetration.
                Available gauges: 26 & 22.



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