This wizard is used when there is more than one sample or reagent that needs to be diluted. For example if an assay requires a sample, internal standard, modifier and buffer to be combine in precise volumes this wizard would be ideal. The system would be primed with the buffer and then the other components of the reaction would be aspirated into the hand probe. An optional air gap can be used to minimize carryover between aspirations. Once everything has been aspirated they are all dispensed. Then the disposable tip can be ejected and the buffer can be dispensed to bring the mixture up to the final volume. The total volume of the samples and air gaps must be less than or equal to the volume of the pipette tip used (50 µL or 1 mL). When using this wizard the appropriate tip volume needs to be selected, this may be done from the Run screen.