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PRP-3

Reversed-phase column optimized for separation of macromolecules such as DNA, RNA oligos, proteins, peptides and proteomics

Hamilton’s PRP-3 is a polymeric reversed-phase HPLC column designed for the purification and isolation of proteins and peptides with very good recovery (> 90%). It is based off of the PRP-1 but utilizes a 300 Å pore size rather than the PRP-1’s 100 Å pore size. The highly inert polymeric packing poly(styrene-divinylbenzene) enhances protein recovery because there are no silanol groups on the support to cause irreversible protein adsorption.

 

PRP-3 is a PS-DVB support that is pressure stable up to 5,000 psi and cross-linked to prevent shrinking or swelling when the mobile phase is changed. Chemically, proteins present solubility problems unlike many small molecules. Most proteins are hydrophobic on the inside, with highly charged exteriors. This often presents dissolution problems, particularly when pH is near the isoelectric point of the protein. The rugged chemical nature of the PRP-3 allows the protein chemist a much broader selection of agents for dissolution, including concentrated acids, aggressive chaotropes, as well as detergents. In proteomics, the PRP-3 has excellent potential for single-column 2D HPLC. The orthogonal selectivity between low and high pH separations is often comparable to that achieved with two different column formats (SCX, RP), but with the added bonus of MS-compatible mobile phase.

 

PRP-3 stationary phase structure and applications

Applications:

Organic compounds: large molecules (> 2,000 mw), peptides, proteins, protein digests, protected and de-protected oligonucleotides, nucleic acids

 

Examples of analytes that can be separated on PRP-3 columns:

  • Globular proteins
  • Albumins
  • Antibody fragments
  • Tryptic digests
  • DNA
  • RNA oligomers
  • Synthetic high mw polymers

Displaying 1 - 20 of 28 products

Part Number/REF  Description  Unit Price  Quantity  
79477 Analytical Guard Cartridge Holder, PEEK $158.00 Add to Cart +
32908 Analytical Guard Cartridge Holder, Stainless Steel $103.00 Add to Cart +
79295 Analytical Guard Column, PRP-3 $267.00 Add to Cart +
79641 Analytical HPLC Column, CUSTOM ORDER (1.0, 2.1, 4.1, 4.6 mm ID) $813.00 Add to Cart +
79643 Preparative HPLC Column #1, CUSTOM ORDER (21.2, 30 mm ID) $3,818.00 Add to Cart +
79644 Preparative HPLC Column #2, CUSTOM ORDER (50 mm ID) Quote Required Add to Cart +
79645 Preparative HPLC Column #3, CUSTOM ORDER (100 mm ID) Quote Required Add to Cart +
79526 PRP-3 10 µm 300 Å 10 x 250 mm $1,536.00 Add to Cart +
79392 PRP-3 10 µm 300 Å 2.1 x 150 mm $496.00 Add to Cart +
79147 PRP-3 10 µm 300 Å 21.2 x 250 mm $2,835.00 Add to Cart +
79466 PRP-3 10 µm 300 Å 4.1 x 150 mm $496.00 Add to Cart +
79794 PRP-3 10 µm 300 Å 4.1 x 250 mm $603.00 Add to Cart +
79382 PRP-3 10 µm 300 Å 4.6 x 150 mm PEEK $543.00 Add to Cart +
79574 PRP-3 10 µm 300 Å 4.6 x 250 mm PEEK $650.00 Add to Cart +
79468 PRP-3 10 µm 300 Å 7.0 x 305 mm $839.00 Add to Cart +
79186 PRP-3 12-20 µm 300 Å 21.2 x 100 mm $1,773.00 Add to Cart +
79469 PRP-3 12-20 µm 300 Å 21.2 x 250 mm $2,540.00 Add to Cart +
79395 PRP-3 Analytical Guard Column Replacement Cartridges (5/pk), PEEK $211.00 Add to Cart +
79454 PRP-3 Analytical Guard Column Replacement Cartridges (5/pk), Stainless Steel $189.00 Add to Cart +
79393 PRP-3 Analytical Guard Column Starter Kit (1 holder, 2 cartridges), PEEK $241.00 Add to Cart +
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