General, chemical type or category of compound to be separated.
The type of resin that is packed inside the column tube.
United States Pharmacopeia HPLC column designation.
Size (in micrometers) of the polymer or silica bead packed inside the column tube.
Inside diameter (in millimeters) of the column tube.
Length of the column tube (in millimeters).
Hamilton guard columns protect your analytical HPLC column. They remove particulate contaminants and highly adsorptive compounds from samples, prolonging analytical column life.
Guard columns are modular and cartridge replacement is easy and tool free. Guard column cartridge holders are available in stainless steel and PEEK materials. Both designs are optimized to reduce dead volume and prevent extra column band broadening.
Holders for the cartridges are reusable indefinitely. Technical specifications and an exploded drawing are shown below for each type of guard column and holder. As with all guard columns these should be installed before the analytical column and after the injection valve.
Guard Columns for Stainless Steel HPLC Columns
Guard Columns for PEEK HPLC Columns
Selecting a Guard Column Packing
Choose a guard column cartridge with the same packing as your analytical HPLC Column.
Conditioning The Guard Column
After installing the guard column cartridge into the holder with the flow arrow pointing toward the analytical column, pump approximately 2 mL of mobile phase through the cartridge before connecting it to the analytical HPLC column.
Sealing the Guard Cartridges
Both guard cartridge holders are designed to seal with finger-tight force. If the guard column leaks, loosen the holder and re-tighten. If the leak persists, remove the cartridge and inspect the cartridge and holder for signs of damage or wear. Replace any damaged or worn parts and reassemble.