General, chemical type or category of compound to be separated.
The type of resin that is packed inside the column tube.
United States Pharmacopeia HPLC column designation.
Size (in micrometers) of the polymer or silica bead packed inside the column tube.
Inside diameter (in millimeters) of the column tube.
Length of the column tube (in millimeters).
The separation of carbohydrates by anion exchange at basic pH is possible because each carbohydrate carries a different negative charge at basic pH. To utilize the full potential of the RCX-10 column (gradient separations) a Pulsed Amperometric Detector (PAD) is recommended. A PAD allows utilization of both gradient and isocratic elution for the separation of carbohydrates. A typical mobile phase is sodium hydroxide and sodium acetate. When the concentration of the mobile phase is changed, a variety of samples can be separated. Application #98 illustrates the gradient separation of carbohydrates in Jerusalem Artichoke Tubers. Note the wide range of oligomers separated (DP1 to DP17). When used for isocratic separations a conductivity, refractive index, ultraviolet or pulsed amperometric detector can be used to quickly determine mono and disaccharides.
Polymeric anion exchange packing for isocratic or gradient separation of carbohydrates