Easy Ion Determination
Hamilton PRP-X100 ion chromatography columns are the easy way to separate inorganic ions. You can use these columns with any existing HPLC or ion chromatograph to easily determine anions in almost any sample matrix including: air, water and soil. PRP-X100 columns provide a number of benefits.

Better than Wet Chemical Methods
Anyone using wet chemical or colorimetric methods will find that ion chromatography greatly reduces sample pretreatment and improves the accuracy and precision of results.

High Sensitivity
Use Hamilton ion chromatography columns to separate ions at concentrations from 10 ppb to 500 ppm without suppression or elaborate preconcentration techniques.

Long Column Life
The highly inert polymeric support resists chemical attack from organic solvents and aqueous buffers (0-100% aqueous or organic; pH 1 to 13), effectively lengthening column life. If column performance should deteriorate (peak broadening and a loss of symmetry), the regeneration protocol will usually return your chromatography to its original state.

Analyze Both Difficult and Common Anions
PRP-X100 columns easily separate difficult anions such as cyanide, borate and silicate at high pH (11.5). The polymeric packing is stable from pH 1 to 13, so a single column can be used for the analysis of both common (see Application #94) and difficult anions (see Application #95 and Application #66).

Easy Detection and Common Eluents
Single column or chemically non-suppressed ion chromatography uses ionized organic acids for eluents (mobile phases). These provide a strong counter-ion for elution and have a low equivalent conductance, allowing conductivity detection to be utilized without chemical suppression. An advantage of indirect UV detection is that an existing variable wavelength UV detector can be used. Since this is a very common detector, in many cases it saves the cost of a specialized detector (conductivity or electrochemical). Indirect UV (vacancy) detects anions by measuring the lack of absorbance as they elute. Once the column is equilibrated, the absorbance is zeroed and the leads from the detector to the integrator are reversed. This allows the negative anion peaks with no absorbance to appear as quantitative positive peaks. Conductivity detection works by measuring the increased conductance of the eluting sample ions relative to the background conductance. Application #95 and #77 illustrate the separation of five anions. They differ only in the detection. Application #95 uses conductivity detection and Application #77 utilizes indirect UV (vacancy) detection. The only difference between the two is the system peak (negative) in the conductivity detection (Application #95). Table 1 lists recommended mobile phases in increasing elution strength.

Polymeric anion exchange packing for separation of inorganic and organic anions from 10 to 500 ppm

• Easily separate the eight common anions (fluoride through sulfate)

• Good separation of fluoride from the water dip

• Use with organic solvent from 0 to 100% for elution of hydrophobic anions or column cleaning

• pH stable from 1 to 13

• Use conductivity or UV detection

 
Product Features

• Three particle sizes: 5, 10 and 12-20 µm

• Custom hardware dimensions available

• Two column materials: 316 stainless steel and PEEK

• Analytical and semiprep/preparative guard column

-----------------

Tips for Anion Exchange Chromatography using the PRP-X100

Ion Exchange Chromatography is a process that allows the separation of ions and polar molecules based on their charge properties. It can be used for almost any kind of charged molecule including inorganic ions, proteins, nucleotides and amino acids.  It is often used in protein purification, water analysis, and quality control.

PRP-X100 Inorganic Anion Analysis Columns

Ion chromatography is most frequently used for the separation and quantitation of inorganic anions. The eight anions most frequently analyzed are fluoride, carbonate, chloride, nitrite, bromide, nitrate, phosphate, and sulfate.  PRPX-100 columns provide excellent resolution of all eight anions (see Application #94).

Controlling Anion Retention

Anion retention and analysis time can be altered by changing eluent strength and flow rate. When the mobile phase concentration is increased from 4 to 6x10^-3 M p-hydroxybenzoic acid, pH 8.5, anion retention is shortened and some resolution is lost. Conversely, if the analysis requires more resolution and greater sample retention, then a 3x10^-3 M p-hydroxybenzoic acid pH 8.5 eluent is more appropriate.

Detection

Detection of anions is generally via electronically suppressed conductivity or indirect UV.  Selecting a detection method is often dictated by the available equipment.  Both conductivity and indirect UV provide comparable detection limits, 0.5 to 150 ppm per anion at a 100 µL injection volume. See Table 1. for mobile phases, detection methods, and anions separated.

Conductivity

Conductivity detection is often the preferred mode because it is the most universal; ionized samples are detected against the poorly conducting mobile phase ions.

Indirect UV

Indirect UV detection works because inorganic ions absorb less in the mid UV (280-350 nm) than mobile phase ions. Sample ions appear as negative peaks in the UV absorbing background.  When the leads from the detector to the recorder are reversed, the peaks appear as positive.

Changing Mobile Phases and Column Equilibration

Equilibration of the PRP-X100 column requires approximately 150 column volumes of mobile phase or 100, 200, 300, and 500 mL for the 50, 100, 150, and 250 mm columns, respectively.  Equilibration is complete when analyte retention is reproducible.

All PRP-X100 columns are shipped in 4 mM p-hydroxybenzoic acid with 2.5% methanol pH 8.5.

Anion chromatography is a sensitive technique.  If chromatographic resolution is inadequate or if you have questions, call the Hamilton Company (1.800.648.5950, 775.858.3000 or email hplc@hamiltoncompany.com).

• Hamilton HPLC Column Compound Index

• Hamilton HPLC Column and Bulk Resin Catalog - Two Page Spread View

• Bulk Polymer Packing Restoration

• Hamilton HPLC Column Instructions

• Influence of Mobile Phase on Retention of Anion Columns (PRP-X100)

• Flexible Anion Analysis

• Arsenic Speciation on PRP-X100

• Reproducibility Anion IC Columns

• New Hamilton Anion Columns