Hamilton HxSil C18 columns exhibit greater retention than most columns. This allows the separation of compounds that are not sufficiently retained on other C18 columns. Figures 1 and 2 illustrate the separation of polycyclic aromatic hydrocarbons (PAH’s) on an HxSil C18 column versus a Nova-Pak® C18 column of the same length. The flow rate has been reduced for the Nova-Pak C18 column to compensate for the smaller I.D. The Hamilton HxSil C18 column provides about twice the retention for these compounds (23 minute run time versus 11 minutes). The greater retention of the HxSil C18 column provides better separation of these PAH compounds.  With the HxSil C18 column, peaks 2 through 6 (naphthalene, acenaphthylene, acenaphthene, phenanthrene, anthracene) are baseline resolved. The Nova-Pak C18 column does not fully separate peaks 2 and 3; peaks 5 and 6 are only partially resolved. Peaks 9 and 10 are better separated on the Hamilton HxSil C18 column.

Silica-based columns for general separations

• Separation of closely related PAH's

• Better retention of poorly retained compounds

• Separation of neutral, acidic and basic compounds without tailing

  (tailing factor of less than 2.0).

Product Features

• Two particle sizes: 3 and 5 µm.

• Custom hardware dimensions available

• Analytical and semiprep/preparative guard columns in 316 stainless steel.

• Hamilton HPLC Column Instructions

• Hamilton Silica Columns

• Hamilton HPLC Column Compound Index

• HxSil C8 and C18 HPLC Columns

• Column Restoration Procedure

• HPLC Resin Specifications