Hamilton Company | Hamilton HPLC Columns & Accessories

HxSil C8

Hamilton HxSil C8 columns are well end-capped for retention time reproducibility and durability. The C8 demonstrates excellent selectivity needed for complex sample mixtures and gives shorter retention times than a C18.

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Compound Class

General, chemical type or category of compound to be separated.

Packing Material

The type of resin that is packed inside the column tube.

USP Class

United States Pharmacopeia HPLC column designation.

Particle Size

Size (in micrometers) of the polymer or silica bead packed inside the column tube.

Hardware ID

Inside diameter (in millimeters) of the column tube.

Hardware Length

Length of the column tube (in millimeters).

The Hamilton HxSil C8 column meets USP L7 requirements.

Antibiotics
The separation of oxytetracycline and tetracycline using an HxSil C8 column is shown in Figure 7.

Steroids
Four steroids are separated on a Hamilton HxSil C8 (Figure 12) column. Excellent selectivity is demonstrated (baseline separation of all four steroids). Two of the steroids, 4-androstene-3,17-dione (peak 2) and testosterone (peak 3) differ only by the oxidation state at the C-17 position; a carbonyl and hydroxyl group respectively. Reduced run times are observed with a C8 column versus a C18 (4.5 minute run time with a C8 versus 7.5 with a C18).

Silica-based columns for general separations

Separation of closely related PAH's
Better retention of poorly retained compounds
Separation of neutral, acidic and basic compounds without tailing

(tailing factor of less than 2.0).

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