Compound ClassGeneral, chemical type or category of compound to be separated. Packing MaterialThe type of resin that is packed inside the column tube. USP ClassUnited States Pharmacopeia HPLC column designation. Particle SizeSize (in micrometers) of the polymer or silica bead packed inside the column tube. Hardware IDInside diameter (in millimeters) of the column tube. Hardware LengthLength of the column tube (in millimeters). Hamilton HxSil C18 columns exhibit greater retention than most columns. This allows the separation of compounds that are not sufficiently retained on other C18 columns. Figures 1 and 2 illustrate the separation of polycyclic aromatic hydrocarbons (PAH’s) on an HxSil C18 column versus a Nova-Pak® C18 column of the same length. The flow rate has been reduced for the Nova-Pak C18 column to compensate for the smaller I.D. The Hamilton HxSil C18 column provides about twice the retention for these compounds (23 minute run time versus 11 minutes). The greater retention of the HxSil C18 column provides better separation of these PAH compounds. With the HxSil C18 column, peaks 2 through 6 (naphthalene, acenaphthylene, acenaphthene, phenanthrene, anthracene) are baseline resolved. The Nova-Pak C18 column does not fully separate peaks 2 and 3; peaks 5 and 6 are only partially resolved. Peaks 9 and 10 are better separated on the Hamilton HxSil C18 column.
Silica-based columns for general separations - Separation of closely related PAH'sBetter retention of poorly retained compounds Product Features - Two particle sizes: 3 and 5 µm.Five column internal diameters: 2.1 to 21.5 mm.
Catalog 
Instruction Sheet
Polymer Restoration
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HPLC Columns
