Hamilton Company | Hamilton HPLC Columns & Accessories

PRP-h5

Hamilton PRP-h5 penta fluorinated polymeric HPLC columns with 300 Å pore size particles. More hydrophilic backbone for peptide and protein separations.

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Part Number

Description

Unit Price

Quantity

Compound Class

General, chemical type or category of compound to be separated.

Packing Material

The type of resin that is packed inside the column tube.

USP Class

United States Pharmacopeia HPLC column designation.

Particle Size

Size (in micrometers) of the polymer or silica bead packed inside the column tube.

Hardware ID

Inside diameter (in millimeters) of the column tube.

Hardware Length

Length of the column tube (in millimeters).

Polymeric 300 Å columns for separation of proteins, peptides and enhanced recovery of oligonucleotides.

pH Stable from 1 to 13

Excellent durability (stable to any concentration of water or organic solvent)

Lower back pressure operation compared to other polymer HPLC columns

Product Features

One particle size: 5 µm

Custom hardware dimensions available

Analytical and semiprep/preparative guard columns

Reproducibility

Hamilton PRP-h5 reversed phase columns are ideal for applications that require a high degree of column-to-column and batch to batch consistency (i.e., quality assurance, clinical trials). Unlike C8 or C18 columns, PRP-h5 has no stationary phase coating. The integral reversed phase characteristics of the PRP-h5 column eliminate the need for special coating techniques. Since there is no stationary phase to hydrolyze, the column maintains its performance characteristics longer than many C8 or C18 columns. While many column manufacturers rely on other sources for their raw silica support, Hamilton manufactures its PRP-h5 support, thus assuring a consistent supply of PRP-h5 columns.

Long Life (Excellent Durability)

PRP-h5 columns are packed with a poly(styrene-divinylbenzene) material. This support is pressure stable up to 5,000 psi, and highly cross-linked to prevent shrinkage or swelling (common in other polymeric columns). Since the support is polymeric, column lifetime is longer for PRP-h5 than C4, C8 or C18 columns. Regardless of whether you use ion-pair reagents or high percentage aqueous buffers (80-100%) for your separation, PRP-h5 columns outlast silica-based columns under most conditions. PRP-h5 columns will last approximately one year, while C8 and C18 columns will last only 3-4 months.