Reversed Phase
	Hamilton Company offers four polymeric and two silica-based column packing materials for reversed phase separations. Choose from our robust polymers or silica column types to narrow your search.
Anion Exchange
	Six polymeric column packings for the separation of inorganic and organic anions, DNA and carbohydrates
Cation Exchange
	Seven polymeric column packings for separation of inorganic and organic cations, glyphosate, organic acids, and sugars.
Ion Exclusion
	PRP-X300 is a polymeric column packing for the separation of alcohols and organic acids
Custom HPLC Column Sizes
	Don't see the column you want? --- If you don't see the column size you require for a particular packing material, you can create your own. (See the Detailed Description tab below)

Roll the cursor over the menu headers below to see the available selections. Filter by one or more items in any order to narrow your search down to a single part number in this category. Reset a filter by clicking the red X that appears next to a filtered item. For more detail on any menu item, click the question mark on the right-hand side of the menu bar. Part Number Description Unit Price Quantity

Hamilton offers 19 different polymer-based HPLC columns for reversed phase, anion exchange, cation exchange and ion exclusion separations.  Two silica-based C8 and C18 column materials are available for reversed phase separations.

Reversed Phase

PRP-1	General purpose pH stable long life column, synthesized DNA
PRP-3	Gradient peptide and protein separations
PRP-h1	Long life column for LC/MS applications,synthesized DNA, and small molecule
PRP-h5	Reduced system pressure for protein and peptide separations and enhanced oligonucleotide recovery
HxSil C8, C18	General purpose silica-based reversed phase

Anion Exchange

PRP-X100	Anions, inorganic and organic using conductivity or UV detection.  0 to 100% solvent compatible.
PRP-X110	Similar to PRP-X100 but for lower level anions (20 ppb to 20 ppm)
PRP-X500	Gradient separation of large proteins and labeled DNA
PRP-X600	Gradient separation of labeled and unlabeled DNA
RCX-10	Isocratic or gradient separation of carbohydrate oligomers up to DP8
RCX-30	Gradient separation of complex carbohydrates

Cation Exchange

PRP-X200	Inorganic and organic cations using conductivity or UV detection.  Separate mono or divalent cations depending on mobile phase conditions.
PRP-X400	Glyphosate and metabolite in drinking water. Also unique hydrophilic interaction separations
PRP-X800	Mono and divalent cations in the same run. Transition metals
HC-40	Sugar oligomers up to DP8. Max pressure 1,000 psi
HC-75 Ca2+	Mono and disaccharides in corn syrup. Max pressure 1,000 psi
HC-75 H+	Organic acids and sugars. Max pressure 1,000 psi
HC-75 Pb2+	Sugar alcohols. Max pressure 1,000 psi

Ion Exclusion

PRP-X300	Organic acids and alcohols

Polymeric Column Benefits

Hamilton's polymeric HPLC columns combine the inertness and pH stability of polymeric supports with the pressure stability and durability of silica-based columns.  Column packings are available for reversed phase, anion exchange, cation exchange, and ion exclusion separations.  There are a total of 19 different packings.

pH Stability

Hamilton's polymeric supports are stable from pH 1 to 13, allowing you to separate samples when they are ionized and more easily detected by UV.  Weak acid anions are also easily separated at high pH without column degradation.

Better Sample Recovery

Polymeric supports do not have silanol groups that can cause irreversible sample retention.  You cannot afford to lose valuable sample on the column. Polymeric columns provide you with the best possible sample recovery.  One example is the purification of DNA oligomers.  Silica-based columns provide sample recoveries of 50-80%, while Hamilton polymeric columns provide recoveries of greater than 90%.

Consistent Performance

Hamilton Company manufactures its own supports.  This allows us to control every aspect of the support synthesis and column packing, ensuring reproducible performance, batch after batch.

Scale-up

Columns are available in a variety of sizes and packed with a variety of particle sizes. This allows you to optimize sample scale-up from 4.1 mm to 10 mm or 50.8 mm sizes.

Excellent Durability

The polymeric columns Hamilton Company manufactures are exceptionally durable and highly cross-linked to prevent shrinkage and swelling (found in other polymeric columns).  These polymeric supports are compatible with o to 100% organic solvent and water. One customer has published a column clean-up method to remove proteins bound to the column from incomplete sample clean-up. It includes flushing with 0.1 N sodium hydroxide and heating the column to 100° C in an oven for three hours. Column resolution was the same as before fouling.

 

 

 

Catalog

• Hamilton HPLC Column Compound Index

• Hamilton HPLC Column and Bulk Resin Catalog - Two Page Spread View

Polymer Restoration

• Bulk Polymer Packing Restoration

Instruction Sheet

• Hamilton HPLC Column Instructions

Custom Column Sizes

Part Number

Description

Unit Price

Quantity

79642

Semipreparative HPLC Column, CUSTOM ORDER (7.0, 7.8, 10 mm ID)

1995.00

79643

Preparative HPLC Column #1, CUSTOM ORDER (21.2, 30 mm ID)

3780.00

79644

Preparative HPLC Column #2, CUSTOM ORDER (50 mm ID)

RFQ

79645

Preparative HPLC Column #3, CUSTOM ORDER (100 mm ID)

RFQ

79641

Analytical HPLC Column [single], CUSTOM ORDER (1.0, 2.1, 4.1, 4.6 mm ID)

805.00