General, chemical type or category of compound to be separated.
The type of resin that is packed inside the column tube.
United States Pharmacopeia HPLC column designation.
Size (in micrometers) of the polymer or silica bead packed inside the column tube.
Inside diameter (in millimeters) of the column tube.
Length of the column tube (in millimeters).
Hamilton offers 19 different polymer-based HPLC columns for reversed phase, anion exchange, cation exchange and ion exclusion separations. Two silica-based C8 and C18 column materials are available for reversed phase separations.Reversed Phase
Polymeric Column Benefits
Hamilton's polymeric HPLC columns combine the inertness and pH stability of polymeric supports with the pressure stability and durability of silica-based columns. Column packings are available for reversed phase, anion exchange, cation exchange, and ion exclusion separations. There are a total of 19 different packings.
Hamilton's polymeric supports are stable from pH 1 to 13, allowing you to separate samples when they are ionized and more easily detected by UV. Weak acid anions are also easily separated at high pH without column degradation.
Better Sample Recovery
Polymeric supports do not have silanol groups that can cause irreversible sample retention. You cannot afford to lose valuable sample on the column. Polymeric columns provide you with the best possible sample recovery. One example is the purification of DNA oligomers. Silica-based columns provide sample recoveries of 50-80%, while Hamilton polymeric columns provide recoveries of greater than 90%.
Hamilton Company manufactures its own supports. This allows us to control every aspect of the support synthesis and column packing, ensuring reproducible performance, batch after batch.
Columns are available in a variety of sizes and packed with a variety of particle sizes. This allows you to optimize sample scale-up from 4.1 mm to 10 mm or 50.8 mm sizes.
Excellent DurabilityThe polymeric columns Hamilton Company manufactures are exceptionally durable and highly cross-linked to prevent shrinkage and swelling (found in other polymeric columns). These polymeric supports are compatible with o to 100% organic solvent and water. One customer has published a column clean-up method to remove proteins bound to the column from incomplete sample clean-up. It includes flushing with 0.1 N sodium hydroxide and heating the column to 100° C in an oven for three hours. Column resolution was the same as before fouling.