A fast, versatile HPLC column packing material, Hamilton
PRP-X300 columns rapidly and easily separate closely related alcohols
and organic acids without column heating. The sulfonated
poly(styrene-divinylbenzene) support separates samples by a mixed mode
The three separation modes are:
1. Hydrogen Bonding
The attraction and retention of sample compounds by the negatively charged sulfonate group
2. Reversed Phase
The interaction and retention of the sample compounds by the nonpolar polymeric support
3. Ion Exclusion
The process in which ionized samples are excluded from the
pores of the support and elute first, while the non-ionic compounds
The dominant separation mechanism is determined by sample composition and separation conditions. In Application #53 a combination of ion exclusion and reversed phase modes perform the separation of wine acids.
In Application #43 hydrogen bonding and reversed phase modes dominate the separation of nine closely related alcohols.
The combination of a powerful mixed mode separation mechanism
and a high performance polymeric support provides benefits that include:
Wide Applicability (Versatility)
A wide range of samples can be analyzed with PRP-X300 columns because
the selectivity of the column can be altered by changing the pH of the
buffer (see table below) or adding an organic modifier (Methanol,
Acetonitrile) to the buffer. The supports stability to organic solvents
makes it possible to analyze samples that have been too highly retained
on conventional ion exclusion supports. Application #41
illustrates the effect of organic solvent on the separation of
2,3-Butanediol and Acetoin. The separation is complete ih 3 1/2 minutes
with 20% Methanol. Without the methanol the separation takes seven to
The excellent selectivity of the PRP-X300 columns ensures most separations are complete within five minutes. In Application #43,
the separation of nine closely related alcohols is complete in five
minutes. The high performance packing ensures narrow peaks under
isocratic conditions. In Application #109, the separation of six keto
acids in four minutes. The use of isocratic conditions allows samples
to be analyzed without column reequilibration. When samples are too
complex or the isocratic analysis takes too long, you can always run a
Easy Sample Preparation
Most samples require only minimal preparation before injection. For the fruit juice sample in Application #114 all that was necessary was to filter and dilute before injection. This helps to increase sample throughput.
illustrates the separation of 2-methoxyethanol and two of its
metabolites. This application is of interest because 2-methoxyethanol
and particularly it's metabolites, are toxic or can cause health
Application #44 illustrates the unique ability of PRP-X300 columns to separate very closely related samples.
k' Sulfuric Acid Mobile Phase Concentration x 10-3 N
Propionic Acid, Butyric Acid, Keto Acids
Elution of these and other more hydrophobic acids require the addition of at least 40% methanol to the mobile phase.
Polymeric ion exclusion packing for
separation of alcohols and organic acids
structurally similar alcohols.
similar organic acids.
organic solvent from 0 to 100% for elution of highly retained compounds.
particle size: 7 µm.
column internal diameters: 1.0 to 4.6 mm.
materials: 316 stainless steel and PEEK
and semiprep/preparative guard columns.
Hamilton Ion Exclusion HPLC Column Packing Materials