Madin-Darby Bovine Kidney cells (MDBK)
Mardin-Darby Bovine Kidney (MDBK) cells have been successfully cultured using the BioLevitator. Listed below are the products required for culturing MDBK cells.
Abstract
Mardin-Darby Bovine Kidney (MDBK) cells were successfully cultivated in the BioLevitator™. The ideal inoculation density was determined to be 2 million cells per 0.5 mL GEM™. Cell viability was higher that 90%. After amplification yields higher than 75 million cells in 30 mL cultures were reached. This represents the amount of cells obtained from seven to eight T75-flasks.
Introduction
The BioLevitator is a unique benchtop incubator and bioreactor hybrid. The unit is a self-contained, all-in-one incubator and centrifuge. It utilizes a unique microcarrier called the GEM, developed by Global Cell Solutions, to facilitate cell growth and expansion of adherent cell lines. Here we report the successful culture of Mardin-Darby Bovine Kidney (MDBK) cells in the BioLevitator. |
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Conclusion
The BioLevitator appeared to be an efficient and straightforward tool for the culture of MDBK cells. Standard yields reached were about 75 million cells from a 30 mL culture, which represents the amount of cells obtained from
seven to eight T75-flasks. In addition, we were able to reduce manipulation time, reagents and consumables required as cells were passaged about 3-times less often. |
Click the "Downloads and Links" tab for more details about the culture protocol and select the file titled Culturing MDBK Cells in the BioLevitator.
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