Automating the growth and maintenance of a Caco-2 cell line helps to ensure that the culture is free from contamination and greatly improves consistency. Hamilton Robotics has developed a method designed to process various trans-well plate formats on the Microlab STAR Automated Liquid Handling Workstation. The entire system is housed in a BSL-2 (biosafety level 2) enclosure, ensuring culture sterility and user safety. Hamilton’s air displacement-based pipetting virtually eliminates contamination. Hamilton Robotics has successfully worked with and automated numerous customer processes, including many unique cell culture growth applications.