How Does a Dispenser Work?
Dispenses, including titrations, can be made in two easy steps (after priming the instrument) as shown in Figure 1.
Figure 1: A simple, dual dispense
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| Step 1: Fill the syringe(s) with the programmed amount of reagent from the reservoir. |
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| Step 2: Dispense the programmed amount(s) into a microwell plate, test tube, vial, etc. to complete the dispense cycle. |
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In addition to simple dispenses performed in all MICROLAB 500 dispensers, models ML510B, ML540B, ML511C, ML541C and ML560C can also perform aliquot, serial, pipette and titrate dispenses. See Figure 2.
ML 500 Series
| Method Type |
A |
B |
C |
Method Illustration |
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| Simple dispense |
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Reagent is filled from a reservoir and dispensed |
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| Aliquot dispense |
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Repetitive dispense with constant final volume |
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| Serial dispense |
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• |
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Repetitive dispense with a variable final volume |
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| Pipette |
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Aspirate sample, then dispense |
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| Titrate |
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Repetitive dispense to endpoint |
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Reagent dilution
(dual syringe only) |
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Addition of two reagents to a common vial |
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| Return to reservoir |
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Save reagent in the fluid path by returning to reagent reservoir |
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Figure 2:
Dispense method types
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| • Blue = diluent |
• Yellow = sample |
• Green = diluted sample |
• Purple = titration endpoint |
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