Simple dilutions can be made in three easy steps (after priming the instrument) as shown in Figure 1:
Figure 1: A simple dilution
Step 1: Fill the left syringe with the programmed amount of solvent (diluent) from the reservoir.
Step 2: Aspirate the programmed amount of concentrated sample into the end of the probe using the right syringe.
Step 3: Dispense the sample and solvent into a vial to complete the dilution.
In addition to simple dilutions performed on all MICROLAB 500 diluters, models ML530B, ML531C and ML560C instruments can also perform serial, and multi-sample/reagent (or internal standard addition) dilutions. See Figure 2.
ML 500 Series
Method Type
A
B
C
Method Illustration
1. Simple dilution
2. Pipette
(w/disposable tip)
•
•
•
Serial dilution
(programmed)
•
•
Serial dilution
(tube to tube)
•
•
Multi-sample/reagent dilution
(or internal standard addition)
•
•
Return to reservoir
•
•
Figure 2:
Dilution method types
• Blue = diluent
• Yellow = sample
• Orange = standard
• Green = diluted sample
• Purple = dilutes sample + standard
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