How Does a Diluter Work?

Simple dilutions can be made in three easy steps (after priming the instrument) as shown in Figure 1:



Figure 1: A simple dilution
Step 1: Fill the left syringe with the programmed amount of solvent (diluent) from the reservoir.
Step 2: Aspirate the programmed amount of concentrated sample into the end of the probe using the right syringe.
Step 3: Dispense the sample and solvent into a vial to complete the dilution.


In addition to simple dilutions performed on all MICROLAB 500 diluters, models ML530B, ML531C and ML560C instruments can also perform serial, and multi-sample/reagent (or internal standard addition) dilutions. See Figure 2.


ML 500 Series

Method Type A B C    Method Illustration
1. Simple dilution
2. Pipette

(w/disposable tip)
Serial dilution
(programmed)
 
Serial dilution
(tube to tube)
 
Multi-sample/reagent dilution
(or internal standard addition)
 
Return to reservoir  


Figure 2:
Dilution method types

 Blue = diluent  Yellow = sample  Orange = standard  Green = diluted sample  Purple = dilutes sample + standard

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