How Does a Diluter Work?
Simple dilutions can be made in three easy steps (after priming the instrument) as shown in Figure 1:
Figure 1: A simple dilution
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| Step 1: Fill the left syringe with the programmed amount of solvent (diluent) from the reservoir. |
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| Step 2: Aspirate the programmed amount of concentrated sample into the end of the probe using the right syringe. |
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| Step 3: Dispense the sample and solvent into a vial to complete the dilution. |
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In addition to simple dilutions performed on all MICROLAB 500 diluters, models ML530B, ML531C and ML560C instruments can also perform serial, and multi-sample/reagent (or internal standard addition) dilutions. See Figure 2.
ML 500 Series
| Method Type |
A |
B |
C |
Method Illustration |
|
1. Simple dilution
2. Pipette
(w/disposable tip) |
• |
• |
• |
 |
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Serial dilution
(programmed) |
|
• |
• |
 |
|
Serial dilution
(tube to tube) |
|
• |
• |
 |
|
Multi-sample/reagent dilution
(or internal standard addition) |
|
• |
• |
 |
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| Return to reservoir |
|
• |
• |
 |
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Figure 2:
Dilution method types
|
| • Blue = diluent |
• Yellow = sample |
• Orange = standard |
• Green = diluted sample |
• Purple = dilutes sample + standard |
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